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Team:Tokyo Metropolitan/Notebook/S26
From 2011.igem.org
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Making LB plate with 0.2~1.5% agar | Making LB plate with 0.2~1.5% agar | ||
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Latest revision as of 15:59, 5 October 2011
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-Targeting-
@plasmid extraction and restriction enzyme(XbaⅠ, PstⅠ) Anti-killer Device-no.6(pSB1C3), Anti-killer Device-no.7(pSB1C3), J23100-P0140(J61002), AHL inducible Device(pSB1C3), E0240, I13522
@electrophoresis
find binds each sample
@gel extraction
J23100-P0140(J61002), AHL inducible Device(pSB1C3), E0240, I13522
@ligation and transformation
J23100-P0140(J61002) and pSB1C3, I13522 and pSB1C3, AHL inducible Device-no1,2(pSB1C3) and E0240
-visualization-
@restriction emzyme oriTr-GFP EcoRⅠ, SpeⅠ
@restriction emzyme oriT EcoRⅠ, XbaⅠ
@restriction emzyme oriTr-GFP SpeⅠ, PstⅠ
@restriction emzyme oriT XbaⅠ, PstⅠ
@restriction emzyme oriTr-GFP-oriTr
-Speeding up-
Preparation for Speeding up device assay
Making LB plate with 0.2~1.5% agar
Colony PCR
