Team:Tokyo Metropolitan/Notebook/S26

From 2011.igem.org

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@ligation and transformation<Br>
@ligation and transformation<Br>
J23100-P0140(J61002) and pSB1C3, I13522 and pSB1C3, AHL inducible Device-no1,2(pSB1C3) and E0240
J23100-P0140(J61002) and pSB1C3, I13522 and pSB1C3, AHL inducible Device-no1,2(pSB1C3) and E0240
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= -visualization- =
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@restriction emzyme oriTr-GFP EcoRⅠ, SpeⅠ
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@restriction emzyme oriT EcoRⅠ, XbaⅠ
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@restriction emzyme oriTr-GFP SpeⅠ, PstⅠ
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@restriction emzyme oriT XbaⅠ, PstⅠ
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@restriction emzyme oriTr-GFP-oriTr
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= -Speeding up- =
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Preparation for Speeding up device assay
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Making LB plate with 0.2~1.5% agar
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Colony PCR

Latest revision as of 15:59, 5 October 2011

-Targeting-

@plasmid extraction and restriction enzyme(XbaⅠ, PstⅠ) Anti-killer Device-no.6(pSB1C3), Anti-killer Device-no.7(pSB1C3), J23100-P0140(J61002), AHL inducible Device(pSB1C3), E0240, I13522

@electrophoresis
find binds each sample

@gel extraction
J23100-P0140(J61002), AHL inducible Device(pSB1C3), E0240, I13522

@ligation and transformation
J23100-P0140(J61002) and pSB1C3, I13522 and pSB1C3, AHL inducible Device-no1,2(pSB1C3) and E0240


-visualization-

@restriction emzyme oriTr-GFP EcoRⅠ, SpeⅠ

@restriction emzyme oriT EcoRⅠ, XbaⅠ

@restriction emzyme oriTr-GFP SpeⅠ, PstⅠ

@restriction emzyme oriT XbaⅠ, PstⅠ

@restriction emzyme oriTr-GFP-oriTr


-Speeding up-

Preparation for Speeding up device assay

Making LB plate with 0.2~1.5% agar

Colony PCR

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