Team:Tokyo Metropolitan/Notebook/A30
From 2011.igem.org
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= -Visualization- = | = -Visualization- = | ||
Plasmid extraction and purification to get J23100・I714051,but there was enough plasmid. | Plasmid extraction and purification to get J23100・I714051,but there was enough plasmid. | ||
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+ | ------------------------------------ | ||
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+ | @plasmid extraction OriTr | ||
+ | |||
+ | @DNA refine OriTr | ||
+ | |||
+ | @DNA densitometry | ||
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+ | @restriction emzyme(EcoRⅠ and SpeⅠ, ScoRⅠ and XbaⅠ) OriTr,OriTr+GFP |
Revision as of 13:55, 5 October 2011
-Speeding Up-
Amplified HNS(wt),I20260 with PCR and purified these PCR products.
-Visualization-
Plasmid extraction and purification to get J23100・I714051,but there was enough plasmid.
@plasmid extraction OriTr
@DNA refine OriTr
@DNA densitometry
@restriction emzyme(EcoRⅠ and SpeⅠ, ScoRⅠ and XbaⅠ) OriTr,OriTr+GFP