Team:Tokyo Metropolitan/Notebook/A30

From 2011.igem.org

(Difference between revisions)
(-Speeding Up-)
Line 8: Line 8:
= -Visualization- =
= -Visualization- =
Plasmid extraction and purification to get J23100・I714051,but there was enough plasmid.
Plasmid extraction and purification to get J23100・I714051,but there was enough plasmid.
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@plasmid extraction OriTr
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@DNA refine OriTr
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@DNA densitometry
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@restriction emzyme(EcoRⅠ and SpeⅠ, ScoRⅠ and XbaⅠ) OriTr,OriTr+GFP

Revision as of 13:55, 5 October 2011

-Speeding Up-

Amplified HNS(wt),I20260 with PCR and purified these PCR products.

-Visualization-

Plasmid extraction and purification to get J23100・I714051,but there was enough plasmid.


@plasmid extraction OriTr

@DNA refine OriTr

@DNA densitometry

@restriction emzyme(EcoRⅠ and SpeⅠ, ScoRⅠ and XbaⅠ) OriTr,OriTr+GFP