Latest revision as of 13:22, 5 October 2011

-Speeding Up-

Extracted plasmid from E.coli transformated BBa_J04450,

Refined by phenol chloroform mixture and ethanol precipitation,

Added restriction enzyme,

HNS(wt)→X,S

BBa_J04450→X,S

@plasmid extraction and DNA refine J23100-P0440

@colony PCR J23100-K317037 and J23100-P0440

@electrophoresis
J23100-K317037: find binds in 29 and 31
J23100-P0440: find binds almost all

@preculture
J23100-K317037 and J23100-P0440 which find binds.

-Ligation

Promoter - suppressed expression system

@@ Line 1: / Line 1: @@
-=Speeding Up=
+{{:Team:Tokyo_Metropolitan/Header}}
-Plasmid extraction and purification (pSB1C3).
+<html>
-Digest HNS(wt) and pSB1C3 with XbaI and SpeI.
+<div style="width: 800px; margin-left: 70px; padding-top: 25px; padding-left: 20px;">
+</html>
+= -Speeding Up- =
+Extracted plasmid from E.coli transformated BBa_J04450,
+Refined by phenol chloroform mixture and ethanol precipitation,
+Added restriction enzyme,
+HNS(wt)→X,S
+BBa_J04450→X,S
+= -Targetting- =
+@plasmid extraction and DNA refine J23100-P0440
+@colony PCR J23100-K317037 and J23100-P0440
+@electrophoresis<Br>
+J23100-K317037: find binds in 29 and 31<Br>
+J23100-P0440: find binds almost all<Br>
+@preculture<Br>
+J23100-K317037 and J23100-P0440 which find binds.
+= -Visualization- =
+-Ligation
+Promoter - suppressed expression system