Team:Tokyo Metropolitan/Notebook/S19

From 2011.igem.org

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=Speeding Up=
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{{:Team:Tokyo_Metropolitan/Header}}
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Member
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Matsumura
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Method
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= -Targeting- =
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Electrophoresis for Hns and parts.
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1. Suspended 1 µl loading buffer and 2 µl sample on parafilm.
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@electrophoresis P0140, pSB1C3, John K Kennedy, GFP<Br>
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2. Put gel in the electrophoresis tank. Poured marker and samples into well.
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find binds all sample but GFP
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3. Electrophoresed for 30 minuets at 100V. Dyed for 10 minuets by ethidium bromide (EtBr).
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4. Took a phot under UV light and checked bands.
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Solution of parts made bands, but solution of Hns did not make bands.
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@gel extraction P0140, John K Kennedy, pSB1C3
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@restriction enzyme(XbaⅠ,Pst) P0140, John K Kennedy, pSB1C3
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@electrophoresis P0140, John K Kennedy, pSB1C3<Br>
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find binds all
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@gel extraction P0140, John K Kennedy, pSB1C3
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@ligation and transformation P0140 and J23100, pSB1C3 and John K Kennedy<Br>
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for contarol, J23100 only and John K Kennedy only

Latest revision as of 12:44, 5 October 2011

-Targeting-

@electrophoresis P0140, pSB1C3, John K Kennedy, GFP
find binds all sample but GFP

@gel extraction P0140, John K Kennedy, pSB1C3

@restriction enzyme(XbaⅠ,Pst) P0140, John K Kennedy, pSB1C3

@electrophoresis P0140, John K Kennedy, pSB1C3
find binds all

@gel extraction P0140, John K Kennedy, pSB1C3

@ligation and transformation P0140 and J23100, pSB1C3 and John K Kennedy
for contarol, J23100 only and John K Kennedy only

Retrieved from "http://2011.igem.org/Team:Tokyo_Metropolitan/Notebook/S19"