Latest revision as of 12:30, 5 October 2011

-Speeding Up-

PCR HNS(wt), BBa_I20260(except GFP) and electrophored.

PCR was worked 98℃ 10sec, 49℃ 15sec, 72℃ 3.5min for 30cycles.

As a result, confirmated increase of HNS(wt) but not BBa_I20260.

HNS(wt) was refined by phenol chloroform mixture and ethanol precipitation.

Preculture Precultured promoter(J23100) and expression inhibiter(I714051)

@electrophoresis

@preculture

OriTR(J01003)

@DNA refine J23200, K317037 and P0440

@@ Line 5: / Line 5: @@
 = -Speeding Up- =
-Amplified HNS(wt) and BBa_I20260 with PCR. Only HNS(wt) was succeeded.
+PCR HNS(wt), BBa_I20260(except GFP) and electrophored.
+PCR was worked 98℃ 10sec, 49℃ 15sec, 72℃ 3.5min for 30cycles.
+As a result, confirmated increase of HNS(wt) but not BBa_I20260.
+HNS(wt) was refined by phenol chloroform mixture and ethanol precipitation.
-Purification of HNS(wt)
 = -Visualization- =
-Preculture(J23100 and I714051)
+Preculture
+Precultured promoter(J23100) and  expression inhibiter(I714051)
@@ Line 18: / Line 25: @@
 OriTR(J01003)
+= -Targetting- =
+@DNA refine J23200, K317037 and P0440