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Team:Tokyo Metropolitan/Notebook/A29
From 2011.igem.org
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| - | + | PCR HNS(wt), BBa_I20260(except GFP) and electrophored. | |
| - | + | ||
| + | PCR was worked 98℃ 10sec, 49℃ 15sec, 72℃ 3.5min for 30cycles. | ||
| + | |||
| + | As a result, confirmated increase of HNS(wt) but not BBa_I20260. | ||
| + | |||
| + | HNS(wt) was refined by phenol chloroform mixture and ethanol precipitation. | ||
| + | |||
| + | = -Visualization- = | ||
| + | |||
| + | Preculture | ||
| + | Precultured promoter(J23100) and expression inhibiter(I714051) | ||
| + | |||
| + | |||
| + | |||
| + | @electrophoresis | ||
| + | |||
| + | @preculture | ||
| + | |||
| + | OriTR(J01003) | ||
| + | |||
| + | = -Targetting- = | ||
| + | |||
| + | @DNA refine J23200, K317037 and P0440 | ||
Latest revision as of 12:30, 5 October 2011
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-Speeding Up-
PCR HNS(wt), BBa_I20260(except GFP) and electrophored.
PCR was worked 98℃ 10sec, 49℃ 15sec, 72℃ 3.5min for 30cycles.
As a result, confirmated increase of HNS(wt) but not BBa_I20260.
HNS(wt) was refined by phenol chloroform mixture and ethanol precipitation.
-Visualization-
Preculture Precultured promoter(J23100) and expression inhibiter(I714051)
@electrophoresis
@preculture
OriTR(J01003)
-Targetting-
@DNA refine J23200, K317037 and P0440
