"
Team:Tokyo Metropolitan/Notebook/S01
From 2011.igem.org
(Difference between revisions)
(→Speeding Up) |
(→-Visualization-) |
||
| (3 intermediate revisions not shown) | |||
| Line 3: | Line 3: | ||
<div style="width: 900px; margin-left: 20px; padding-top: 25px; padding-left: 20px;"> | <div style="width: 900px; margin-left: 20px; padding-top: 25px; padding-left: 20px;"> | ||
</html> | </html> | ||
| - | =Speeding Up= | + | = -Speeding Up- = |
Made agar plates | Made agar plates | ||
Made each five agar plates contained kanamycin and chloramphenicol. | Made each five agar plates contained kanamycin and chloramphenicol. | ||
| - | = - | + | |
| + | = -Visualization- = | ||
Digestion,electrophoresis and gel extraction J23100,I714051. | Digestion,electrophoresis and gel extraction J23100,I714051. | ||
| Line 13: | Line 14: | ||
Concentration of DNA solution(J23100,I714051) will measure tomorrow. | Concentration of DNA solution(J23100,I714051) will measure tomorrow. | ||
| + | |||
| + | |||
| + | |||
| + | @DNA refine(OriTr+GFP,OriT) | ||
| + | |||
| + | electrophoresis and gel extract restriction enzymed sample(8/31). | ||
| + | |||
| + | |||
| + | @ligation | ||
| + | |||
| + | considering a small quantity of refined DNA, change reagent composition. | ||
| + | |||
| + | |||
| + | I. ligate solution | ||
| + | |||
| + | oriTr:1μl | ||
| + | |||
| + | oriTr+GFP:12μl | ||
| + | |||
| + | T4：1μl | ||
| + | |||
| + | ×10 buffer：2μl | ||
| + | |||
| + | |||
| + | II. control solution | ||
| + | |||
| + | DW:12μl | ||
| + | |||
| + | oriTr:1μl | ||
| + | |||
| + | T4：1μl | ||
| + | |||
| + | ×10 buffer：2μl | ||
Latest revision as of 11:13, 5 October 2011
 |
 |
 |
 |
 |
 |
 |
 |
-Speeding Up-
Made agar plates
Made each five agar plates contained kanamycin and chloramphenicol.
-Visualization-
Digestion,electrophoresis and gel extraction J23100,I714051.
Confirm plasmid extraction was succeeded
Concentration of DNA solution(J23100,I714051) will measure tomorrow.
@DNA refine(OriTr+GFP,OriT)
electrophoresis and gel extract restriction enzymed sample(8/31).
@ligation
considering a small quantity of refined DNA, change reagent composition.
I. ligate solution
oriTr:1μl
oriTr+GFP:12μl
T4：1μl
×10 buffer：2μl
II. control solution
DW:12μl
oriTr:1μl
T4：1μl
×10 buffer：2μl
