Team:Tokyo Metropolitan/Notebook/S01

From 2011.igem.org

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= -Speeding Up- =
Made agar plates
Made agar plates
Made each five agar plates contained kanamycin and chloramphenicol.
Made each five agar plates contained kanamycin and chloramphenicol.
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= -Visualization- =
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Digestion,electrophoresis and gel extraction J23100,I714051.
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Confirm plasmid extraction was succeeded
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Concentration of DNA solution(J23100,I714051) will measure tomorrow.
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@DNA refine(OriTr+GFP,OriT)
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electrophoresis and gel extract restriction enzymed sample(8/31).
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@ligation
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considering a small quantity of refined DNA, change reagent composition.
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I. ligate solution
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　oriTr:1μl
 +
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　oriTr+GFP:12μl
 +
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　T4：1μl
 +
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　×10 buffer：2μl
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II. control solution
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　DW:12μl
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　oriTr:1μl
 +
 +
　T4：1μl
 +
 +
　×10 buffer：2μl

Latest revision as of 11:13, 5 October 2011

-Speeding Up-

Made agar plates

Made each five agar plates contained kanamycin and chloramphenicol.

-Visualization-

Digestion,electrophoresis and gel extraction J23100,I714051.

Confirm plasmid extraction was succeeded

Concentration of DNA solution(J23100,I714051) will measure tomorrow.


@DNA refine(OriTr+GFP,OriT)

electrophoresis and gel extract restriction enzymed sample(8/31).


@ligation

considering a small quantity of refined DNA, change reagent composition.


I. ligate solution

　oriTr:1μl

　oriTr+GFP:12μl

　T4：1μl

　×10 buffer：2μl


II. control solution

　DW:12μl

　oriTr:1μl

　T4：1μl

　×10 buffer：2μl

Retrieved from "http://2011.igem.org/Team:Tokyo_Metropolitan/Notebook/S01"