Team:Macquarie Australia

From 2011.igem.org

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<p style="font: 11pt Trebuchet MS">G'day from the 2011 Macquarie University iGEM group. <br><br> This year's research team will be expanding upon the research conducted by last year's iGEM team. This project involves the production of a phytochrome light switch that jumps between two different light states, acting as a reporter for ambient light conditions. We've nicknamed our work 'the switch-a-roo' as our phytochromes hop back and forth between green and blue states.</p>
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<p style="font: 11pt Trebuchet MS">Hello from the 2011 Macquarie University iGEM group! <br><br> This year's research team will be expanding upon the research conducted by last year's iGEM team. This project involves the production of a phytochrome light switch that jumps between two different light states, acting as a reporter for ambient light conditions. We've nicknamed our work 'the switch-a-roo' as our phytochromes hop back and forth between green and blue states.</p>
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Here are some quick links to help you get started: <br>
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Here are some quick links to help you get started: <br><br>
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<p style="font: 11pt Trebuchet MS"> Photoreceptors are ubiquitous proteins that allow an organism to sense light. These proteins have evolved in unique environments to sense light intensity in different colour ranges. This experiment focuses on constructing a biological switch that uses two photoreceptors from separate organisms – Deinococcus radiodurans and Agrobacterium tumafaciens. The coupling of heme oxygenase supplies our photoreceptor proteins with biliverdin, allowing for the self-assembly of the switch within host systems. The switch is the first stage of a two component light sensor and when expressed at high level, there is a noticeable colour change of the cell when it is activated by light.</p>
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<p style="font: 11pt Trebuchet MS"> Phytochromes are ubiquitous proteins that allow an organism to sense light. These proteins have evolved in unique environments to sense light intensity in different colour ranges. This experiment focuses on constructing a biological switch that uses phytochromes from Deinococcus radiodurans and Agrobacterium tumefaciens. The coupling of heme oxygenase supplies our phytochrome proteins with biliverdin, allowing for the self-assembly of the switch within host systems. The switch is the first stage of a two component light sensor and when expressed at high level, there is a noticeable colour change of the cell when it is activated by light.</p>
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<td class="title"><center>Bronze</td>
<td class="title"><center>Bronze</td>
<td class="middletitle"><center>Silver</td>
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<td><a href="https://igem.org/Team.cgi?id=646"><img src="http://governmentconstructioncontracts.com/images/checkbox_green-checkmark.gif">Registration of Team</a></td>
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<td><center><a href="https://igem.org/Team.cgi?id=646"><img src="https://online.slimmingworld.com/images/registration/packages/tick-bronze.jpg">Registration of Team</a></td>
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<td class="middle"><center><a href="https://2011.igem.org/Team:Macquarie_Australia/Characterisation#Characterisation_of_our_Biobrick"><img src="https://online.slimmingworld.com/images/registration/packages/tick-silver.jpg">Characterisation of our working Biobrick</a></td>
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<td class="middle"><a href="https://2011.igem.org/Team:Macquarie_Australia/Characterisation"><img src="http://governmentconstructioncontracts.com/images/checkbox_green-checkmark.gif">Characterisation of our working Biobrick</a></td>
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<td class="far"><center><a href="https://2011.igem.org/Team:Macquarie_Australia/parts#Improvement_of_previous_BioBrick"><img src="http://secremedia-hosting.co.uk/images/gold-tick.png">Improvement of previous Biobricks
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<td><a href="https://igem.org/2011_Judging_Form?id=646"><img src="http://governmentconstructioncontracts.com/images/checkbox_green-checkmark.gif">Judging form</a></td>
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<td class="middle"><a href="https://2011.igem.org/Team:Macquarie_Australia/parts"><img src="http://governmentconstructioncontracts.com/images/checkbox_green-checkmark.gif">Information entered onto Main Page of Registry </a></td>
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<td class="middle"><center><a href="https://2011.igem.org/Team:Macquarie_Australia/parts"><img src="https://online.slimmingworld.com/images/registration/packages/tick-silver.jpg">Information entered onto Main Page of Registry </a></td>
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<td><img src="http://governmentconstructioncontracts.com/images/checkbox_green-checkmark.gif">Project wiki</td>
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<td><center><img src="https://online.slimmingworld.com/images/registration/packages/tick-bronze.jpg">Project wiki</td>
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<td><img src="http://governmentconstructioncontracts.com/images/checkbox_green-checkmark.gif">Poster and Talk for Asia Jamboree</td>
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<td><center><img src="https://online.slimmingworld.com/images/registration/packages/tick-bronze.jpg">Poster and Talk for Asia Jamboree</td>
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<td><a href="https://2011.igem.org/Team:Macquarie_Australia/parts"><img src="http://governmentconstructioncontracts.com/images/checkbox_green-checkmark.gif">Submission of parts to Registry</td></tr>
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<td><center><a href="https://2011.igem.org/Team:Macquarie_Australia/parts"><img src="https://online.slimmingworld.com/images/registration/packages/tick-bronze.jpg">Submission of parts to Registry</td></tr>
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<td><img src="http://governmentconstructioncontracts.com/images/checkbox_green-checkmark.gif">Submission of Biobrick to Registry [number needed]</td>
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<td><center><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K646000"><img src="https://online.slimmingworld.com/images/registration/packages/tick-bronze.jpg">Submission of Biobrick to Registry</td>
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Latest revision as of 02:08, 5 October 2011




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Welcome


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Hello from the 2011 Macquarie University iGEM group!

This year's research team will be expanding upon the research conducted by last year's iGEM team. This project involves the production of a phytochrome light switch that jumps between two different light states, acting as a reporter for ambient light conditions. We've nicknamed our work 'the switch-a-roo' as our phytochromes hop back and forth between green and blue states.


Here are some quick links to help you get started:

  • An overview of our Project

  • Head on over to our Data Page for a summary of our registered parts

  • Or meet the Team!

  • The requirements for each medal grade can be seen at the bottom of this page


Abstract


Phytochromes are ubiquitous proteins that allow an organism to sense light. These proteins have evolved in unique environments to sense light intensity in different colour ranges. This experiment focuses on constructing a biological switch that uses phytochromes from Deinococcus radiodurans and Agrobacterium tumefaciens. The coupling of heme oxygenase supplies our phytochrome proteins with biliverdin, allowing for the self-assembly of the switch within host systems. The switch is the first stage of a two component light sensor and when expressed at high level, there is a noticeable colour change of the cell when it is activated by light.



Medal Progress
Retrieved from "http://2011.igem.org/Team:Macquarie_Australia"