Team:UQ-Australia/Data

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[HERE] Summary [HERE]
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Revision as of 16:16, 4 October 2011




A brief summary of some of our laboratory findings is included below. For more information, see the 'project' page. IGEM basic Logo stylized.png
UQ-Australia logo 2011.png

We conducted a heat cycle PCR to determine the optimum conditions for amplifying our araC gene. The lanes here are loaded as follows:

1: 1kb+ ladder

2: Negative control (H2O)

3-7: pBAD33 plasmid at temperatures 50C, 55C, 58C, 60C and 65C.

8: Negative control (H2O)

9-13: pBAD33 plasmid at temperatures 50C, 55C, 58C, 60C and 65C.

AraCpcr.png

PCR of the lacI gene

IMG 0194.JPG

PCR of glnG (lanes 2 and 3), glnAp2 (lanes 5 and 6), and the promoter Plac/ara (lanes 6-8 of the second gel). Only glnG is the expected size.

IMG 0195.JPG