Team:UQ-Australia/Data

(Difference between revisions)

Revision as of 12:20, 4 October 2011

There is a '[HERE]'where you should edit in places where I remembered to put them. [HERE] Summary [HERE]

We conducted a heat cycle PCR to determine the optimum conditions for amplifying our araC gene. The lanes here are loaded as follows:

1: 1kb+ ladder

2: Negative control (H2O)

3-7: pBAD33 plasmid at temperatures 50C, 55C, 58C, 60C and 65C.

8: Negative control (H2O)

9-13: pBAD33 plasmid at temperatures 50C, 55C, 58C, 60C and 65C.

PCR of the lacI gene

PCR of glnG (lanes 2 and 3), glnAp2 (lanes 5 and 6), and the promoter Plac/ara (lanes 6-8 of the second gel). Only glnG is the expected size.

@@ Line 26: / Line 26: @@
 [[File:IMG_0194.JPG‎ | 231x125px]]
-PCR of glnG (lanes 2 and 3), glnAp2 (lanes 5 and 6), and the promoter Plac/ara (lanes 6-8 of the second gel)
+PCR of glnG (lanes 2 and 3), glnAp2 (lanes 5 and 6), and the promoter Plac/ara (lanes 6-8 of the second gel). Only glnG is the expected size.
-[[File:IMG_0195.JPG | 300x190px]]
+[[File:IMG_0195.JPG | 400x250px]]