Team:Osaka/week8

From 2011.igem.org

(Difference between revisions)
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# Transformation of ligation products
# Transformation of ligation products
# Plating (cell survival)
# Plating (cell survival)
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{|style="border-collapse: separate; border-spacing: 0; border-width: 1px; border-style: solid; border-color: #000; padding:5px;" cellpadding="5"
 
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|+Table 10
 
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!Name!!OD600!!UV(mJ/cm^2)
 
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|-
 
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|PprI-1||0.0109||0
 
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|-
 
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|PprI-2||0.0109||0.5
 
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|-
 
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|PprI-3||0.0109||1.0
 
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|-
 
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|PprI-4||0.0109||2.0
 
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|-
 
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|PprI-5||0.0109||5.0
 
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|-
 
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|PprA-1||0.0418||0
 
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|-
 
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|PprA-2||0.0418||0.5
 
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|-
 
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|PprA-3||0.0418||1.0
 
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|-
 
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|PprA-4||0.0418||2.0
 
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|-
 
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|PprA-5||0.0418||5.0
 
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|-
 
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|PprM-1||0.0454||0
 
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|-
 
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|PprM-2||0.0454||0.5
 
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|-
 
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|PprM-3||0.0454||1.0
 
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|-
 
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|PprM-4||0.0454||2.0
 
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|-
 
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|PprM-5||0.0454||5.0
 
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|-
 
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|RecA-1||0.0114||0
 
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|-
 
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|RecA-2||0.0114||0.5
 
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|-
 
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|RecA-3||0.0114||1.0
 
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|-
 
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|RecA-4||0.0114||2.0
 
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|-
 
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|RecA-5||0.0114||5.0
 
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|-
 
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|empty vector-1||0.0515||0
 
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|-
 
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|empty vector-2||0.0515||0.5
 
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|-
 
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|empty vector-3||0.0515||1.0
 
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|-
 
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|empty vector-4||0.0515||2.0
 
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|-
 
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|empty vector-5||0.0515||5.0
 
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|-
 
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|wild type-1||0.0546||0
 
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|-
 
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|wild type-2||0.0546||0.5
 
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|-
 
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|wild type-3||0.0546||1.0
 
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|-
 
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|wild type-4||0.0546||2.0
 
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|-
 
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|wild type-5||0.0546||5.0
 
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|}
 
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# To liquid culture:113,114
# To liquid culture:113,114
# colony check (cell survival)
# colony check (cell survival)
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{|style="border-collapse: separate; border-spacing: 0; border-width: 1px; border-style: solid; border-color: #000; padding:5px;" cellpadding="5"
 
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|+Table 11
 
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!Name!!UV(mJ/cm^2)!!conoly counts
 
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|-
 
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|PprI-1||0||
 
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|-
 
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|PprI-2||0.5||
 
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|-
 
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|PprI-3||1.0||
 
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|-
 
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|PprI-4||2.0||
 
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|-
 
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|PprI-5||5.0||
 
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|-
 
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|PprA-1||0||
 
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|-
 
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|PprA-2||0.5||
 
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|-
 
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|PprA-3||1.0||
 
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|-
 
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|PprA-4||2.0||
 
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|-
 
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|PprA-5||5.0||
 
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|-
 
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|PprM-1||0||
 
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|-
 
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|PprM-2||0.5||
 
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|-
 
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|PprM-3||1.0||
 
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|-
 
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|PprM-4||2.0||
 
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|-
 
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|PprM-5||5.0||
 
-
|-
 
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|RecA-1||0||
 
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|-
 
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|RecA-2||0.5||
 
-
|-
 
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|RecA-3||1.0||
 
-
|-
 
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|RecA-4||2.0||
 
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|-
 
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|RecA-5||5.0||
 
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|-
 
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|Empty vector-1||0||
 
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|-
 
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|Empty vector-2||0.5||
 
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|-
 
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|Empty vector-3||1.0||
 
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|-
 
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|Empty vector-4||2.0||
 
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|-
 
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|Empty vector-5||5.0||
 
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|-
 
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|wild type-1||0||
 
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|-
 
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|wild type-2||0.5||
 
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|-
 
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|wild type-3||1.0||
 
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|-
 
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|wild type-4||2.0||
 
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|-
 
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|wild type-5||5.0||
 
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|}
 
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# Plating (cell survival)
 
-
 
-
 
==September 21(Wed)==
==September 21(Wed)==

Revision as of 13:04, 2 October 2011


Contents

September 18(Sun)

  1. Miniprep of yesterday's culture
  2. Restriction digests of minipreppped parts
  3. Gel electrophoresis of digests
  4. To liquid culture:03,105,106,107,108,109,110,111,112

September 19(Mon)

  1. Miniprep of yesterday's culture
  2. Restriction digests of minipreppped parts
  3. Gel electrophoresis of digests
    • ok
  4. Ligation
    • 113(T):105(upstream)+110(downstream)+1-7A(vector)
    • 114(T):111(upstream)+112(downstream)+1-7A(vector)
  5. Transformation of ligation products
  6. Plating (cell survival)


September 20(Tue)

  1. To liquid culture:113,114
  2. colony check (cell survival)

September 21(Wed)

  1. Miniprep of yesterday's culture
  2. Restriction digests of minipreppped parts
  3. Gel electrophoresis of digests
    • ok
  4. Ligation
    • 115(A):113(upstream)+114(downstream)+1-1G(vector)
  5. Transformation of ligation products
  6. colony check (cell survival)
  7. Plating (cell survival)


September 22(Thu)

  1. Colony check
  2. Plating
  3. To liquid culture

September 23(Fri)

  1. Miniprep of yesterday's culture
  2. Restriction digests of minipreppped parts
  3. Gel electrophoresis of digests
    • ok
  4. Colony check

September 24(Sat)

  1. Preparation of LB plates
  2. Plating


Back to Notebook

week 1|2|3|4|5|6|7|8|9|10

Retrieved from "http://2011.igem.org/Team:Osaka/week8"