Team:Osaka/week6

From 2011.igem.org

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==September 5(Mon)==
==September 5(Mon)==
# Absorbance measurement
# Absorbance measurement
-
{|style="border-collapse: separate; border-spacing: 0; border-width: 1px; border-style: solid; border-color: #000; padding:5px;" cellpadding="5"
 
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|+Table 7
 
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!Name!!IPTG(0.1M)!!incubation!!OD600!!A474
 
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|-
 
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|1|| -||-||0.8583||0.1567
 
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|-
 
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|2||400μl||2h||1.1523||0.1447
 
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|-
 
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|3||400μl||1h||0.5583||0.1126
 
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|-
 
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|4||40μl||2h||1.0297||0.1878
 
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|-
 
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|5||40μl||1h||0.6576||0.1736
 
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|}
 
# To liquid culture:03,17,3-10C
# To liquid culture:03,17,3-10C
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# Transformation of ligation products
# Transformation of ligation products
# Absorbance measurement
# Absorbance measurement
-
{|style="border-collapse: separate; border-spacing: 0; border-width: 1px; border-style: solid; border-color: #000; padding:5px;" cellpadding="5"
 
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|+Table 8
 
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!Name!!UV irradiation!!incubation!!OD600!!A474
 
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|-
 
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|03①|| -||-||  ||
 
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|-
 
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|03②||1mJ/cm2||1h||  ||
 
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|04①|| -||-||  ||
 
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|04②||1mJ/cm2||1h||  ||
 
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==September 10(Sat)==
==September 10(Sat)==
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# PCR purification
# PCR purification
# Absorbance measurement
# Absorbance measurement
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{|style="border-collapse: separate; border-spacing: 0; border-width: 1px; border-style: solid; border-color: #000; padding:5px;" cellpadding="5"
 
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|+Table 9
 
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!Name!!UV irradiation!!incubation!!OD600!!A474
 
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|-
 
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|03①|| -||-||  ||
 
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|-
 
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|03②||1mJ/cm2||1h||  ||
 
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|-
 
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|04①|| -||-||  ||
 
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|-
 
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|04②||1mJ/cm2||1h||  ||
 
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|}
 

Revision as of 13:03, 2 October 2011


Contents

September 4(Sun)

  1. Restriction digests:3-10C,1-2M
  2. Gel extraction of digests
  3. Ligation
    • 03:1-9N+3-6H again
  4. Transformation of ligation products
  5. Photospectrometry measuament

September 5(Mon)

  1. Absorbance measurement
  2. To liquid culture:03,17,3-10C


September 6(Tue)

  1. Miniprep of yesterday's culture
  2. Restriction digests of minipreppped parts
  3. Gel electrophoresis of digests
    • all parts ×
  4. PCR to amplify PprI,PprA,PprM,RecA (DNA repair protein) from D.radiodurance
  5. Gel Electrophoresis of PCR products
    • PprM ok
    • PprI,PprA,RecA ×
  6. PCR
  7. Gel Electrophoresis of PCR products
    • ×


September 7(Wed)

  1. PCR
  2. Gel electrophoresis of PCR products
    • PprM ok
    • PprI,PprA,RecA ×
  3. Gel electrophoresis of PprM
  4. Gel extraction
  5. PCR
  6. Gel electrophoresis of PCR products
    • PprI,PprA,RecA ×
  7. Miniprep of yesterday's culture
  8. Restriction digests of miniprepped parts
  9. Gel electrophoresis of digests
    • 03 ok
  10. To liquid culture:03,04 (preincubation)
  11. PCR
  12. Electrophoresis of PCR products
  13. PCR

September 8(Thu)

  1. Electrophoresis of PCR products
  2. Restriction digests of 1-3A(backbone)
  3. PCR
  4. Electrophoresis of PCR products and miniprepped parts
  5. Gel extraction of 1-3A
  6. PCR
  7. Electrophoresis of PCR products
  8. Inoculation of 03,04
  9. To liquid culture 03,04
  10. PCR
  11. Electrophoresis of PCR products


September 9(Fri)

  1. PCR
  2. Electrophoresis of PCR products
  3. Gel extraction
  4. Restriction digests
  5. PCR
  6. Electrophoresis of PCR products
  7. Gel extraction
  8. Restriction digests
  9. Ligation
    • 101(C):PprI+1-3A(vector)
    • 102(C):PprA+1-3A(vector)
  10. Transformation of ligation products
  11. Absorbance measurement

September 10(Sat)

  1. PCR
  2. Electrophoresis of PCR products
  3. To liquid culture:101,102
  4. PCR
  5. PCR purification
  6. Absorbance measurement


Back to Notebook

week 1|2|3|4|5|6|7|8|9|10

Retrieved from "http://2011.igem.org/Team:Osaka/week6"