Team:Macquarie Australia
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Revision as of 06:17, 30 September 2011
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Welcome
G'day from the 2011 Macquarie University iGEM group.
Here are some quick links to help you get started:
Abstract
The objective in this project is to build and characterise a biological light switch in E.coli. This will involve construction of bacteriophytochrome biobrick parts and heme-oxygenase biobrick parts. In 2010 the Macquarie Team cloned bacteriophytochrome from two sources. They showed that when one was expressed that it was functionally assembled when incubated with biliverdin. The part created is not directly usable as a biobrick as it contains an internal PstI site and the XbaI biobrick site is missing. The heme-oxygenase clone also contains an internal restriction site which is not compatible with biobrick assembly.
Medal Progress
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