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Team:Tokyo Metropolitan
From 2011.igem.org
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Revision as of 10:28, 29 September 2011
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Abstract : BeE.coli
We propose the project "BeE.coli" as a micro injection system.BeE.coli has the abilities to move fast and target pathogens concentrated area.Getting close to pathogens, it sends the killer gene to them with conjugation. Then pathogens will be killed. To accomplish this project, we planned to add three functions to E.coli.First, expression of the mutant H-NS proteins enables E.coli to move faster. This contributes to increasing the frequency of conjugation. Second function is new taxis for AHL. We make the target bacteria as a model of pathogens, and they produce AHL. BeE.coli goes for the area where high concentrations of AHL that means concentration of target bacteria. Last, BeE.coli kills pathogens by sending the killer gene with the conjugative plasmid. BeE.coli has the anti killer gene repressing the killer gene. |
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