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Copenhagen/27 June 2011
From 2011.igem.org
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*Did point mutations - see protocol here: [[Team:Copenhagen/Protocol#Mutations|Mutations]] | *Did point mutations - see protocol here: [[Team:Copenhagen/Protocol#Mutations|Mutations]] | ||
| - | *Used our mutated plasmids from last week to transform competent XL1-Blue cells. Again. But this time we used 10μL of our plasmids instead of 1μL. See protocol here: [[ | + | *Used our mutated plasmids from last week to transform competent XL1-Blue cells. Again. But this time we used 10μL of our plasmids instead of 1μL. See protocol here: [[Team:Copenhagen/Protocol#Transformations|Transformations]] |
Revision as of 14:19, 27 June 2011
Monday
Today was a very dissapointing day. We have not been able to grow any cultures with our CYPs. But we'll try again!
And we are not completely useless cause we have managed to produce competent cells and amplify an ekspression vector from the iGEM kit. Hurra.
Lab Work
- Did point mutations - see protocol here: Mutations
- Used our mutated plasmids from last week to transform competent XL1-Blue cells. Again. But this time we used 10μL of our plasmids instead of 1μL. See protocol here: Transformations
