Team:UTP-Panama/Week 17
From 2011.igem.org
(→WET LAB) |
(→WET LAB) |
||
Line 38: | Line 38: | ||
Miniprep of The RENBO (BBa_K672000) | Miniprep of The RENBO (BBa_K672000) | ||
- | Electrophoresis of The RENBO | + | Electrophoresis of The RENBO BBa_K672000 |
[[File:RENBO 28SEPT2011 TOMA3b.jpg]] | [[File:RENBO 28SEPT2011 TOMA3b.jpg]] | ||
- | The first column shows the reference. The other 4 columns show RENBO. | + | The first column shows the reference. The other 4 columns show The RENBO. |
We obtained the results of our experiment using the Spectrophotometer. With this equipment, we measure the concentration of colonies (optical density) in the different substrates and temperatures. | We obtained the results of our experiment using the Spectrophotometer. With this equipment, we measure the concentration of colonies (optical density) in the different substrates and temperatures. |
Revision as of 05:10, 29 September 2011
Home |
Week 1 | Week 2 | Week 3 | Week 4 | Week 5 | Week 6 | Week 7 | Week 8 | Week 9 | Week 10 | Week 11 | Week 12 | Week 13 | Week 14 | Week 15 | Week 16 | Week 17 | After Regional Week 1 | After Regional Week 2 |
Week 17: September 26 to 28September 26WET LABTransformation of competent cells with Bristol 2010 - BBa_K381001. Electrophoresis to BBa_K672000 – RENBO (sent to the registry) + RENBO (Ligation Product). During this procedure we couldn’t get a good picture of the dna in the transiluminator because there wasn’t enough dna in the samples. We are going to redo this procedure. Experiment DesignWe designed the experiments we are going to do with the three BioBricks used (Bristol 2010 - BBa_K381001, GaTech 2010 – BBa_K410000, UTP-Panama 2011 – BBa_K672000). These temperatures were selected so we could evaluate the response to cold shock in various ranges of our BioBricks. Since the lab equipments we had available couldn’t reach temperatures between 11-20°C this temperature was not used during the experience. Finally, the lab equipment allowed us to make the experiments in: 37°C, 23°C and 8°C.
We also chose 3 different Growth Media: September 27WET LABTransfer of a colony of RENBO into Liquid LB to grow overnight. Preparation of Minimal Media and Saline Solutions for the experiment. We set up the experience designed in a total of 27 15mL Falcon tubes. September 28WET LABMiniprep of The RENBO (BBa_K672000) Electrophoresis of The RENBO BBa_K672000 The first column shows the reference. The other 4 columns show The RENBO. We obtained the results of our experiment using the Spectrophotometer. With this equipment, we measure the concentration of colonies (optical density) in the different substrates and temperatures. |