Team:Cornell/Week 1

From 2011.igem.org

(Difference between revisions)

Latest revision as of 04:00, 29 September 2011

Results | Protocol | Notebook | Parts Submitted

June 5th - June 11th

Sunday, June 5

Monday, June 6

Tuesday, June 7

Cornell iGEM Bootcamp Training Session (Day 1)

Part I

1. PCR setup

2. Run an agarose gel and gel purify the samples using Qiagen Kit.

3. Quantify the samples using NanoDrop.

4. Digest 1μg of DNA sample for 2 hours in the 37°C waterbath for 2 hours.

5. PCR clean up the samples and quantify using NanoDrop.

Part II

1. Miniprep the overnight culture using Qiagen Kit.

2. Quantify the samples using NanoDrop.

3. Digest 1μg of DNA sample for 2 hours in the 37°C water bath for 2 hours.

4. Add CIAP to the backbone and put in the 50°C water bath for 5 minutes.

5. Run the samples on the agarose gel.

6. Cut out the band of right size, gel purify, and quantify with NanoDrop.

Wednesday, June 8

Weill Hall Lab Space Introduction by Dr. Archer

Cornell iGEM Bootcamp Training Session (Day 2)

1. Set up ligation reaction using 50-100ng of backbone and 1:3 ratio of insert to backbone. Put at room temperature for 2 hours.

2. Desalt the ligation samples on a membrane.

3. Electroporate the samples in the electrocompetent cells.

4. Shake the cells in 37°C shaker for 1 hour.

5. Plate the cells onto the agar plate treated with the appropriate antibiotic. Incubate it overnight at 37°C.

Thursday, June 9

Cornell iGEM Bootcamp Training Session (Day 3)

1. Check the colonies on the plates

Friday, June 10

Team Meeting

Organized the new lab space in Weill
Ordered the materials needed for lab work

@@ Line 1: / Line 1: @@
-{{:Team:Cornell/Templates/test}}
+{{:Team:Cornell/Templates/Menu}}
 {{:Team:Cornell/Templates/hideHeader}}
 {{:Team:Cornell/Templates/MediaMenu}}
@@ Line 7: / Line 7: @@
 <font size="5"> <i> June 5th - June 11th </i></font>
 </html><br><br>
-==Sunday==
+==Sunday, June 5==
-==Monday==
+==Monday, June 6==
-==Tuesday==
+==Tuesday, June 7==
-:'''CUgem bootcamp training session (day 1)'''
+:'''Cornell iGEM Bootcamp Training Session (Day 1)'''
 ::'''Part I'''
 :::1. PCR setup
-:::2. Run an agarose gel and Gel purify the samples using Qaigen Kit.
+:::2. Run an agarose gel and gel purify the samples using Qiagen Kit.
-:::3. Quantify the samples using nanodrop.
+:::3. Quantify the samples using NanoDrop.
-:::4. Digest 1μg of DNA sample for 2hours in the 37°C waterbath for 2hours.
+:::4. Digest 1μg of DNA sample for 2 hours in the 37°C waterbath for 2 hours.
-:::5. PCR clean up the samples and quantify using nanodrop.
+:::5. PCR clean up the samples and quantify using NanoDrop.
 ::'''Part II'''
-:::1. Miniprepping the overnight culture using Qaigen Kit.
+:::1. Miniprep the overnight culture using Qiagen Kit.
-:::2. Quantify the samples using nanodrop.
+:::2. Quantify the samples using NanoDrop.
-:::3. Digest 1μg of DNA sample for 2hours in the 37°C waterbath for 2hours.
+:::3. Digest 1μg of DNA sample for 2 hours in the 37°C water bath for 2 hours.
-:::4. Add CIAP to the backbone and put in the 50°C waterbath for 5minutes.
+:::4. Add CIAP to the backbone and put in the 50°C water bath for 5 minutes.
 :::5. Run the samples on the agarose gel.
-:::6. Cut out the band of right size, gel purify and quantify with nanodrop.
+:::6. Cut out the band of right size, gel purify, and quantify with NanoDrop.
-==Wednesday==
+==Wednesday, June 8==
-:'''Weill Hall labspace introduction by Dr.Archer'''
+:'''Weill Hall Lab Space Introduction by Dr. Archer'''
-:'''Cugem bootcamp training session (day 2)'''
+:'''Cornell iGEM Bootcamp Training Session (Day 2)'''
+:::1. Set up ligation reaction using 50-100ng of backbone and 1:3 ratio of insert to backbone. Put at room temperature for 2 hours.
+:::2. Desalt the ligation samples on a membrane.
+:::3. Electroporate the samples in the electrocompetent cells.
+:::4. Shake the cells in 37°C shaker for 1 hour.
+:::5. Plate the cells onto the agar plate treated with the appropriate antibiotic. Incubate it overnight at 37°C.
-==Thursday==
+==Thursday, June 9==
-:'''Cugem bootcamp training session (day 3)'''
+:'''Cornell iGEM Bootcamp Training Session (Day 3)'''
+:::1. Check the colonies on the plates
-==Friday==
+==Friday, June 10==
-:'''Team meeting'''
+:'''Team Meeting'''
-::*Organized the new lab space in Weill.
+::*Organized the new lab space in Weill
-::*Ordered the materials needed for the labwork.
+::*Ordered the materials needed for lab work
-==Saturday==
+==Saturday, June 11==
-__NOTOC__