Team:Cornell/Week 1

From 2011.igem.org

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<font size="4">Week 1 <br><br> June 5th - June 11th </font>
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<font size="5"> <i> June 5th - June 11th </i></font>
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</html><br><br>
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==Sunday==
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==Sunday, June 5==
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==Monday==
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==Monday, June 6==
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==Tuesday==
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==Tuesday, June 7==
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:'''Cornell iGEM Bootcamp Training Session (Day 1)'''
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::'''Part I'''
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:::1. PCR setup
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:::2. Run an agarose gel and gel purify the samples using Qiagen Kit.
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:::3. Quantify the samples using NanoDrop.
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:::4. Digest 1μg of DNA sample for 2 hours in the 37°C waterbath for 2 hours.
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:::5. PCR clean up the samples and quantify using NanoDrop.
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::'''Part II'''
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:::1. Miniprep the overnight culture using Qiagen Kit.
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:::2. Quantify the samples using NanoDrop.
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:::3. Digest 1μg of DNA sample for 2 hours in the 37°C water bath for 2 hours.
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:::4. Add CIAP to the backbone and put in the 50°C water bath for 5 minutes.
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:::5. Run the samples on the agarose gel.
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:::6. Cut out the band of right size, gel purify, and quantify with NanoDrop.
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==Wednesday==
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==Wednesday, June 8==
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:'''Weill Hall Lab Space Introduction by Dr. Archer'''
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==Thursday==
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:'''Cornell iGEM Bootcamp Training Session (Day 2)'''
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:::1. Set up ligation reaction using 50-100ng of backbone and 1:3 ratio of insert to backbone. Put at room temperature for 2 hours.
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:::2. Desalt the ligation samples on a membrane.
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:::3. Electroporate the samples in the electrocompetent cells.
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:::4. Shake the cells in 37°C shaker for 1 hour.
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:::5. Plate the cells onto the agar plate treated with the appropriate antibiotic. Incubate it overnight at 37°C.
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==Friday==
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==Thursday, June 9==
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:'''Cornell iGEM Bootcamp Training Session (Day 3)'''
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:::1. Check the colonies on the plates
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==Saturday==
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==Friday, June 10==
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:'''Team Meeting'''
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::*Organized the new lab space in Weill
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::*Ordered the materials needed for lab work
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__NOTOC__
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==Saturday, June 11==

Latest revision as of 04:00, 29 September 2011

Results | Protocol | Notebook | Parts Submitted

Week 1 | Week 2 - 5 | Week 6 | Week 7 | Week 8 | Week 9 | Week 10 | Week 11 | Week 12 | Week 13 | Week 14 | Week 15 | Week 16 | Week 17 | Week 18 | Week 19 | Week 20 | Week 21 |


June 5th - June 11th

Sunday, June 5

Monday, June 6

Tuesday, June 7

Cornell iGEM Bootcamp Training Session (Day 1)
Part I
1. PCR setup
2. Run an agarose gel and gel purify the samples using Qiagen Kit.
3. Quantify the samples using NanoDrop.
4. Digest 1μg of DNA sample for 2 hours in the 37°C waterbath for 2 hours.
5. PCR clean up the samples and quantify using NanoDrop.
Part II
1. Miniprep the overnight culture using Qiagen Kit.
2. Quantify the samples using NanoDrop.
3. Digest 1μg of DNA sample for 2 hours in the 37°C water bath for 2 hours.
4. Add CIAP to the backbone and put in the 50°C water bath for 5 minutes.
5. Run the samples on the agarose gel.
6. Cut out the band of right size, gel purify, and quantify with NanoDrop.

Wednesday, June 8

Weill Hall Lab Space Introduction by Dr. Archer
Cornell iGEM Bootcamp Training Session (Day 2)
1. Set up ligation reaction using 50-100ng of backbone and 1:3 ratio of insert to backbone. Put at room temperature for 2 hours.
2. Desalt the ligation samples on a membrane.
3. Electroporate the samples in the electrocompetent cells.
4. Shake the cells in 37°C shaker for 1 hour.
5. Plate the cells onto the agar plate treated with the appropriate antibiotic. Incubate it overnight at 37°C.

Thursday, June 9

Cornell iGEM Bootcamp Training Session (Day 3)
1. Check the colonies on the plates

Friday, June 10

Team Meeting
  • Organized the new lab space in Weill
  • Ordered the materials needed for lab work

Saturday, June 11

Retrieved from "http://2011.igem.org/Team:Cornell/Week_1"