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Team:Cornell/Week 1
From 2011.igem.org
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| - | {{:Team:Cornell/Templates/ | + | {{:Team:Cornell/Templates/Menu}} |
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==Tuesday, June 7== | ==Tuesday, June 7== | ||
| - | :''' | + | :'''Cornell iGEM Bootcamp Training Session (Day 1)''' |
::'''Part I''' | ::'''Part I''' | ||
:::1. PCR setup | :::1. PCR setup | ||
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:'''Weill Hall Lab Space Introduction by Dr. Archer''' | :'''Weill Hall Lab Space Introduction by Dr. Archer''' | ||
| - | :''' | + | :'''Cornell iGEM Bootcamp Training Session (Day 2)''' |
:::1. Set up ligation reaction using 50-100ng of backbone and 1:3 ratio of insert to backbone. Put at room temperature for 2 hours. | :::1. Set up ligation reaction using 50-100ng of backbone and 1:3 ratio of insert to backbone. Put at room temperature for 2 hours. | ||
:::2. Desalt the ligation samples on a membrane. | :::2. Desalt the ligation samples on a membrane. | ||
| Line 37: | Line 37: | ||
:::5. Plate the cells onto the agar plate treated with the appropriate antibiotic. Incubate it overnight at 37°C. | :::5. Plate the cells onto the agar plate treated with the appropriate antibiotic. Incubate it overnight at 37°C. | ||
| - | ==Thursday== | + | ==Thursday, June 9== |
| - | :''' | + | :'''Cornell iGEM Bootcamp Training Session (Day 3)''' |
| - | :::1. Check the colonies on the plates | + | :::1. Check the colonies on the plates |
| - | ==Friday== | + | ==Friday, June 10== |
| - | :'''Team | + | :'''Team Meeting''' |
| - | ::*Organized the new lab space in Weill | + | ::*Organized the new lab space in Weill |
| - | ::*Ordered the materials needed for | + | ::*Ordered the materials needed for lab work |
| - | ==Saturday== | + | ==Saturday, June 11== |
| - | + | ||
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Latest revision as of 04:00, 29 September 2011
Results |
Protocol |
Notebook |
Parts Submitted
Week 1 | Week 2 - 5 | Week 6 | Week 7 | Week 8 | Week 9 | Week 10 | Week 11 | Week 12 | Week 13 | Week 14 | Week 15 | Week 16 | Week 17 | Week 18 | Week 19 | Week 20 | Week 21 |
June 5th - June 11th
Sunday, June 5
Monday, June 6
Tuesday, June 7
- Cornell iGEM Bootcamp Training Session (Day 1)
- Part I
- 1. PCR setup
- 2. Run an agarose gel and gel purify the samples using Qiagen Kit.
- 3. Quantify the samples using NanoDrop.
- 4. Digest 1μg of DNA sample for 2 hours in the 37°C waterbath for 2 hours.
- 5. PCR clean up the samples and quantify using NanoDrop.
- Part II
- 1. Miniprep the overnight culture using Qiagen Kit.
- 2. Quantify the samples using NanoDrop.
- 3. Digest 1μg of DNA sample for 2 hours in the 37°C water bath for 2 hours.
- 4. Add CIAP to the backbone and put in the 50°C water bath for 5 minutes.
- 5. Run the samples on the agarose gel.
- 6. Cut out the band of right size, gel purify, and quantify with NanoDrop.
- Part I
Wednesday, June 8
- Weill Hall Lab Space Introduction by Dr. Archer
- Cornell iGEM Bootcamp Training Session (Day 2)
- 1. Set up ligation reaction using 50-100ng of backbone and 1:3 ratio of insert to backbone. Put at room temperature for 2 hours.
- 2. Desalt the ligation samples on a membrane.
- 3. Electroporate the samples in the electrocompetent cells.
- 4. Shake the cells in 37°C shaker for 1 hour.
- 5. Plate the cells onto the agar plate treated with the appropriate antibiotic. Incubate it overnight at 37°C.
Thursday, June 9
- Cornell iGEM Bootcamp Training Session (Day 3)
- 1. Check the colonies on the plates
Friday, June 10
- Team Meeting
- Organized the new lab space in Weill
- Ordered the materials needed for lab work
