Latest revision as of 03:57, 29 September 2011

Home

Team

Project

Official Team Profile

Laboratory Notebook

Brainstorming	Week 1	Week 2	Week 3	Week 4
Week 5	Week 6	Week 7	Week 8	Week 9
Week 10	Week 11	Week 12	Week 13	Week 14

Week 7:

07/19/2011
1. Gel verification for CASO (1-8)
Observation: in second row, GFP spcer, tetR spacer, CRISPR#1, and CRISPR #2
2. Gel verification by digestion
3. Purify tetR from Gel
4. Ligate pSB1C3 with tetR, place in the 15c incubator overnight
5. Transformation

07/21/2011
1. Transform ligations of tetR in DH5α and top10 competent cells
2. Inoculate PCOLA, PCDF and CRISPR

07/22/2011
1. Mini-prep for :
tetR in DH5α and top10 (16 samples)
CRISPR (2 samples)
PCOLA (1 sample)
PCDF (5 samples)
2. Digest tetR with EcoR1 and Pst1
3. Test the digested tetR by running a gel

@@ Line 34: / Line 34: @@
 <table>
 <tr>
-<h1 style="font-family:Verdana;font-weight:700;">Brainstorming</h1>
+<h1 style="font-family:Verdana;font-weight:700;">Laboratory Notebook</h1>
 </tr>
@@ Line 40: / Line 40: @@
 [[Team:USC/Notebook/Brainstorming|Brainstorming]]
 <br/>
-[[File:Brain_storm.JPG]]
+[[File:Brain_storm.JPG|link=Team:USC/Notebook/Brainstorming|Brainstorming]]
 </td>
@@ Line 46: / Line 46: @@
 [[Team:USC/Notebook/Week1|Week 1]]
 <br/>
-[[File:week1.jpg]]
+[[File:week1.jpg|link=Team:USC/Notebook/Week1|Week 1]]
 </td>
@@ Line 52: / Line 52: @@
 [[Team:USC/Notebook/Week2|Week 2]]
 <br/>
-[[File:week2.jpg]]
+[[File:week2.jpg|link=Team:USC/Notebook/Week2|Week 2]]
 </td>
@@ Line 58: / Line 58: @@
 [[Team:USC/Notebook/Week3|Week 3]]
 <br/>
-[[File:week3.jpg]]
+[[File:week3.jpg|link=Team:USC/Notebook/Week3|Week 3]]
 </td>
@@ Line 64: / Line 64: @@
 [[Team:USC/Notebook/Week4|Week 4]]
 <br/>
-[[File:week4.jpg]]
+[[File:week4.jpg|link=Team:USC/Notebook/Week4|Week 4]]
 </td>
@@ Line 72: / Line 72: @@
 [[Team:USC/Notebook/Week5|Week 5]]
 <br/>
-[[File:week5.jpg]]
+[[File:week5.jpg|link=Team:USC/Notebook/Week5|Week 5]]
 </td>
@@ Line 78: / Line 78: @@
 [[Team:USC/Notebook/Week6|Week 6]]
 <br/>
-[[File:week6.jpg]]
+[[File:week6.jpg|link=Team:USC/Notebook/Week6|Week 6]]
 </td>
@@ Line 84: / Line 84: @@
 [[Team:USC/Notebook/Week7|Week 7]]
 <br/>
-[[File:week7.jpg]]
+[[File:week7.jpg|link=Team:USC/Notebook/Week7|Week 7]]
 </td>
@@ Line 90: / Line 90: @@
 [[Team:USC/Notebook/Week8|Week 8]]
 <br/>
-[[File:week8.jpg]]
+[[File:week8.jpg|link=Team:USC/Notebook/Week8|Week 8]]
 </td>
@@ Line 96: / Line 96: @@
 [[Team:USC/Notebook/Week9|Week 9]]
 <br/>
-[[File:week9.jpg]]
+[[File:week9.jpg|link=Team:USC/Notebook/Week9|Week 9]]
 </td>
@@ Line 104: / Line 104: @@
 [[Team:USC/Notebook/Week10|Week 10]]
 <br/>
-[[File:week10.jpg]]
+[[File:week10.jpg|link=Team:USC/Notebook/Week10|Week 10]]
 </td>
@@ Line 110: / Line 110: @@
 [[Team:USC/Notebook/Week11|Week 11]]
 <br/>
-[[File:week11.jpg]]
+[[File:week11.jpg|link=Team:USC/Notebook/Week11|Week 11]]
 </td>
@@ Line 116: / Line 116: @@
 [[Team:USC/Notebook/Week12|Week 12]]
 <br/>
-[[File:week12.jpg]]
+[[File:week12.jpg|link=Team:USC/Notebook/Week12|Week 12]]
 </td>
@@ Line 122: / Line 122: @@
 [[Team:USC/Notebook/Week13|Week 13]]
 <br/>
-[[File:week13.jpg]]
+[[File:week13.jpg|link=Team:USC/Notebook/Week13|Week 13]]
 </td>
@@ Line 128: / Line 128: @@
 [[Team:USC/Notebook/Week14|Week 14]]
 <br/>
-[[File:week14.jpg]]
+[[File:week14.jpg|link=Team:USC/Notebook/Week14|Week 14]]
 </td>
@@ Line 137: / Line 137: @@
 </table>
 <h3 style="font-family:Verdana; font-weight:700;background-color: #F0F0F0;">'''Week 7:'''</h3>