Team:USC/Notebook/Week3
From 2011.igem.org
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- | <span style="float: left; padding: 15px;"><span style="font-family: Arial, Helvetica, sans-serif;font-size: | + | <span style="float: left; padding: 15px;"><span style="font-family: Arial, Helvetica, sans-serif;font-size: 15px;font-weight: bold;color: #FFFFFF;border: none;">[[Team:USC/Parts|Parts]]</span></span> |
- | <span style="float: left; padding: 15px;"><span style="font-family: Arial, Helvetica, sans-serif;font-size: | + | <span style="float: left; padding: 15px;"><span style="font-family: Arial, Helvetica, sans-serif;font-size: 15px;font-weight: bold;color: #FFFFFF;border: none;">[[Team:USC/Safety|Safety]]</span></span> |
- | <span style="float: left; padding: 15px;"><span style="font-family: Arial, Helvetica, sans-serif;font-size: | + | <span style="float: left; padding: 15px;"><span style="font-family: Arial, Helvetica, sans-serif;font-size: 15px;font-weight: bold;color: #FFFFFF;border: none;">[[Team:USC/Human Outreach|Human Outreach]]</span></span> |
- | <span style="float: left; padding: 15px;"><span style="font-family: Arial, Helvetica, sans-serif;font-size: | + | <span style="float: left; padding: 15px;"><span style="font-family: Arial, Helvetica, sans-serif;font-size: 15px;font-weight: bold;color: #FFFFFF;border: none;">[[Team:USC/Attributions|Attributions]]</span></span> |
+ | <span style="float: left; padding: 15px;"><span style="font-family: Arial, Helvetica, sans-serif;font-size: 15px;font-weight: bold;color: #FFFFFF;border: none;">[https://igem.org/Team.cgi?year=2011&team_name=USC Official Team Profile]</span></span> | ||
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+ | <h1 style="font-family:Verdana;font-weight:700;">Laboratory Notebook</h1> | ||
+ | </tr> | ||
<td style="text-align:center; font-family:Verdana; font-weight:700;"> | <td style="text-align:center; font-family:Verdana; font-weight:700;"> | ||
[[Team:USC/Notebook/Brainstorming|Brainstorming]] | [[Team:USC/Notebook/Brainstorming|Brainstorming]] | ||
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- | [[File:Brain_storm.JPG]] | + | [[File:Brain_storm.JPG|link=Team:USC/Notebook/Brainstorming|Brainstorming]] |
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[[Team:USC/Notebook/Week1|Week 1]] | [[Team:USC/Notebook/Week1|Week 1]] | ||
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- | [[File:week1.jpg]] | + | [[File:week1.jpg|link=Team:USC/Notebook/Week1|Week 1]] |
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<td style="text-align:center; font-family:Verdana; font-weight:700;"> | <td style="text-align:center; font-family:Verdana; font-weight:700;"> | ||
[[Team:USC/Notebook/Week2|Week 2]] | [[Team:USC/Notebook/Week2|Week 2]] | ||
<br/> | <br/> | ||
- | [[File:week2.jpg]] | + | [[File:week2.jpg|link=Team:USC/Notebook/Week2|Week 2]] |
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[[Team:USC/Notebook/Week3|Week 3]] | [[Team:USC/Notebook/Week3|Week 3]] | ||
<br/> | <br/> | ||
- | [[File:week3.jpg]] | + | [[File:week3.jpg|link=Team:USC/Notebook/Week3|Week 3]] |
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[[Team:USC/Notebook/Week4|Week 4]] | [[Team:USC/Notebook/Week4|Week 4]] | ||
<br/> | <br/> | ||
- | [[File:week4.jpg]] | + | [[File:week4.jpg|link=Team:USC/Notebook/Week4|Week 4]] |
</td> | </td> | ||
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+ | <tr> | ||
<td style="text-align:center; font-family:Verdana; font-weight:700;"> | <td style="text-align:center; font-family:Verdana; font-weight:700;"> | ||
[[Team:USC/Notebook/Week5|Week 5]] | [[Team:USC/Notebook/Week5|Week 5]] | ||
<br/> | <br/> | ||
- | [[File:week5.jpg]] | + | [[File:week5.jpg|link=Team:USC/Notebook/Week5|Week 5]] |
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[[Team:USC/Notebook/Week6|Week 6]] | [[Team:USC/Notebook/Week6|Week 6]] | ||
<br/> | <br/> | ||
- | [[File:week6.jpg]] | + | [[File:week6.jpg|link=Team:USC/Notebook/Week6|Week 6]] |
</td> | </td> | ||
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<td style="text-align:center; font-family:Verdana; font-weight:700;"> | <td style="text-align:center; font-family:Verdana; font-weight:700;"> | ||
[[Team:USC/Notebook/Week7|Week 7]] | [[Team:USC/Notebook/Week7|Week 7]] | ||
<br/> | <br/> | ||
- | [[File:week7.jpg]] | + | [[File:week7.jpg|link=Team:USC/Notebook/Week7|Week 7]] |
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[[Team:USC/Notebook/Week8|Week 8]] | [[Team:USC/Notebook/Week8|Week 8]] | ||
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- | [[File:week8.jpg]] | + | [[File:week8.jpg|link=Team:USC/Notebook/Week8|Week 8]] |
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[[Team:USC/Notebook/Week9|Week 9]] | [[Team:USC/Notebook/Week9|Week 9]] | ||
<br/> | <br/> | ||
- | [[File:week9.jpg]] | + | [[File:week9.jpg|link=Team:USC/Notebook/Week9|Week 9]] |
</td> | </td> | ||
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<td style="text-align:center; font-family:Verdana; font-weight:700;"> | <td style="text-align:center; font-family:Verdana; font-weight:700;"> | ||
[[Team:USC/Notebook/Week10|Week 10]] | [[Team:USC/Notebook/Week10|Week 10]] | ||
<br/> | <br/> | ||
- | [[File:week10.jpg]] | + | [[File:week10.jpg|link=Team:USC/Notebook/Week10|Week 10]] |
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[[Team:USC/Notebook/Week11|Week 11]] | [[Team:USC/Notebook/Week11|Week 11]] | ||
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[[Team:USC/Notebook/Week12|Week 12]] | [[Team:USC/Notebook/Week12|Week 12]] | ||
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- | [[File:week12.jpg]] | + | [[File:week12.jpg|link=Team:USC/Notebook/Week12|Week 12]] |
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[[Team:USC/Notebook/Week13|Week 13]] | [[Team:USC/Notebook/Week13|Week 13]] | ||
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- | [[File:week13.jpg]] | + | [[File:week13.jpg|link=Team:USC/Notebook/Week13|Week 13]] |
+ | </td> | ||
+ | |||
+ | <td style="text-align:center; font-family:Verdana; font-weight:700;"> | ||
+ | [[Team:USC/Notebook/Week14|Week 14]] | ||
+ | <br/> | ||
+ | [[File:week14.jpg|link=Team:USC/Notebook/Week14|Week 14]] | ||
+ | </td> | ||
+ | |||
+ | </tr> | ||
+ | </table> | ||
</td> | </td> | ||
</tr> | </tr> | ||
- | < | + | </table> |
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<h3 style="font-family:Verdana; font-weight:700;background-color: #F0F0F0;">'''Week 3:'''</h3> | <h3 style="font-family:Verdana; font-weight:700;background-color: #F0F0F0;">'''Week 3:'''</h3> | ||
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Latest revision as of 03:56, 29 September 2011
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Week 3:
06/20/2011
1. Lab meeting
2. Ligate the blunted ended DNA
06/21/2011
1. Column purify the DNA
2. Make buffers for competent cells
Buffer: 10mM KOAc+80mM CaCl2-2H2O+70mM MnCl2-4H2O+10mM MgCl2-6H2O
3. Transformation again for:
Plasmids:OLE1, MSN2, TSP1, ELO1, CAS0, pSB1AK8
Vectors: prs 423-426
06/22/2011
1. Inoculation for :
pRS vectors 423-426 and pSB1AK8
2. PCR ligation products:
OLE1, MSN2, TSP1, ELO1
06/23/2011
1. Transfer E.Coli from agar stabs from iGEM delivery
BBa-K191005
BBa-K191004
BBa-K426020
BBa-I15010
Notes:
+ The agar should already have a hole from when it was stabbed. With an inoculating loop dipped into the stab, you can plate directly
onto a petri dish with the appropriate antibiotic;
+ Incubate the dish overnight at 37 C (14-16hrs)
+Pick a single colony to start up a culture
2. Gel running to test OLE1, MSN2, TSP1, ELO1, CAS0,
Observation: OLE was the only one with a clear band
06/24/2011
1. Observations of the E.Coli transformation from yesterday:
BBa-K191005 and BBa-K191004 are purple
BBa-I15010 is good
06/25/2011
1. Transform for 4 ligated genes: OLE1, MSN2, TSP1, ELO1
Notes: use 5 µL of samples
2. Inoculate for:
BBa-K191005
BBa-K191004
BBa-K426020
BBa-I15010