Team:USC/Notebook/Week3

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<span style="float: left; padding: 15px;"><span style="font-family: Arial, Helvetica, sans-serif;font-size: 11px;font-weight: bold;color: #FFFFFF;border: none;">[[Team:USC|Home]]</span></span>
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<span style="float: left; padding: 15px;"><span style="font-family: Arial, Helvetica, sans-serif;font-size: 15px;font-weight: bold;color: #FFFFFF;border: none;">[[Team:USC|Home]]</span></span>
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                         <span style="float: left; padding: 15px;"><span style="font-family: Arial, Helvetica, sans-serif;font-size: 15px;font-weight: bold;color: #FFFFFF;border: none;">[[Team:USC/Human Outreach|Human Outreach]]</span></span>
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                         <span style="float: left; padding: 15px;"><span style="font-family: Arial, Helvetica, sans-serif;font-size: 15px;font-weight: bold;color: #FFFFFF;border: none;">[https://igem.org/Team.cgi?year=2011&team_name=USC Official Team Profile]</span></span>
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<h1 style="font-family:Verdana;font-weight:700;">Laboratory Notebook</h1>
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<td style="text-align:center; font-family:Verdana; font-weight:700;">
<td style="text-align:center; font-family:Verdana; font-weight:700;">
[[Team:USC/Notebook/Brainstorming|Brainstorming]]
[[Team:USC/Notebook/Brainstorming|Brainstorming]]
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[[File:Brain_storm.JPG]]
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[[File:Brain_storm.JPG|link=Team:USC/Notebook/Brainstorming|Brainstorming]]
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[[Team:USC/Notebook/Brainstorming|Week 1]]
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[[Team:USC/Notebook/Week1|Week 1]]
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[[File:week1.jpg]]
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[[File:week1.jpg|link=Team:USC/Notebook/Week1|Week 1]]
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[[Team:USC/Notebook/Week2|Week 2]]
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[[File:week2.jpg|link=Team:USC/Notebook/Week2|Week 2]]
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[[Team:USC/Notebook/Brainstorming|Week 2]]
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[[Team:USC/Notebook/Week3|Week 3]]
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[[File:week2.jpg]]
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[[File:week3.jpg|link=Team:USC/Notebook/Week3|Week 3]]
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[[Team:USC/Notebook/Brainstorming|Week 3]]
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[[Team:USC/Notebook/Week4|Week 4]]
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[[File:week3.jpg]]
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[[File:week4.jpg|link=Team:USC/Notebook/Week4|Week 4]]
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[[Team:USC/Notebook/Brainstorming|Week 4]]
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[[Team:USC/Notebook/Week5|Week 5]]
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[[File:week4.jpg]]
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[[File:week5.jpg|link=Team:USC/Notebook/Week5|Week 5]]
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[[Team:USC/Notebook/Brainstorming|Week 5]]
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[[Team:USC/Notebook/Week6|Week 6]]
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[[File:week5.jpg]]
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[[File:week6.jpg|link=Team:USC/Notebook/Week6|Week 6]]
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[[Team:USC/Notebook/Brainstorming|Week 6]]
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[[Team:USC/Notebook/Week7|Week 7]]
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[[File:week6.jpg]]
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[[File:week7.jpg|link=Team:USC/Notebook/Week7|Week 7]]
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[[Team:USC/Notebook/Brainstorming|Week 7]]
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[[Team:USC/Notebook/Week8|Week 8]]
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[[File:week7.jpg]]
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[[File:week8.jpg|link=Team:USC/Notebook/Week8|Week 8]]
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[[Team:USC/Notebook/Brainstorming|Week 8]]
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[[Team:USC/Notebook/Week9|Week 9]]
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[[File:week8.jpg]]
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[[File:week9.jpg|link=Team:USC/Notebook/Week9|Week 9]]
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[[Team:USC/Notebook/Brainstorming|Week 9]]
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[[Team:USC/Notebook/Week10|Week 10]]
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[[File:week9.jpg]]
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[[File:week10.jpg|link=Team:USC/Notebook/Week10|Week 10]]
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[[Team:USC/Notebook/Brainstorming|Week 10]]
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[[Team:USC/Notebook/Week11|Week 11]]
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[[File:week10.jpg]]
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[[File:week11.jpg|link=Team:USC/Notebook/Week11|Week 11]]
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[[Team:USC/Notebook/Brainstorming|Week 11]]
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[[Team:USC/Notebook/Week12|Week 12]]
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[[File:week11.jpg]]
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[[File:week12.jpg|link=Team:USC/Notebook/Week12|Week 12]]
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[[Team:USC/Notebook/Brainstorming|Week 12]]
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[[Team:USC/Notebook/Week13|Week 13]]
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[[File:week12.jpg]]
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[[File:week13.jpg|link=Team:USC/Notebook/Week13|Week 13]]
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[[Team:USC/Notebook/Brainstorming|Week 13]]
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[[Team:USC/Notebook/Week14|Week 14]]
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[[File:week13.jpg]]
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[[File:week14.jpg|link=Team:USC/Notebook/Week14|Week 14]]
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<h3 style="font-family:Verdana; font-weight:700;background-color: #F0F0F0;">'''Week 3:'''</h3>
<h3 style="font-family:Verdana; font-weight:700;background-color: #F0F0F0;">'''Week 3:'''</h3>
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Vectors: prs 423-426
Vectors: prs 423-426
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<span style="font-family:Verdana; font-weight:700;"> 06/22/2011 </span>
<span style="font-family:Verdana; font-weight:700;"> 06/22/2011 </span>
<br />
<br />
-
1. 1. Inoculation for :
+
1. Inoculation for :
<br />
<br />
pRS vectors 423-426 and pSB1AK8
pRS vectors 423-426 and pSB1AK8
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<span style="font-family:Verdana; font-weight:700;"> 06/23/2011 </span>
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<br />
 +
1. Transfer E.Coli from agar stabs from iGEM delivery
 +
<br />
 +
BBa-K191005
 +
<br />
 +
BBa-K191004
 +
<br />
 +
BBa-K426020
 +
<br />
 +
BBa-I15010
 +
<br />
 +
Notes:
 +
<br />
 +
+ The agar should already have a hole from when it was stabbed. With an inoculating loop dipped into the stab, you can plate directly
 +
<br />
 +
onto a petri dish with the appropriate antibiotic;
 +
<br />
 +
+ Incubate the dish overnight at 37 C (14-16hrs)
 +
<br />
 +
+Pick a single colony to start up a culture
 +
<br />
 +
2. Gel running to test OLE1, MSN2, TSP1, ELO1, CAS0,
 +
<br />
 +
Observation: OLE was the only one with a clear band
 +
<br />
 +
 
 +
 
 +
<span style="font-family:Verdana; font-weight:700;"> 06/24/2011 </span>
 +
<br />
 +
1. Observations of the E.Coli transformation from yesterday:
 +
<br />
 +
BBa-K191005 and BBa-K191004 are purple
 +
<br />     
 +
BBa-I15010 is good
 +
<br />
 +
 
 +
 
 +
 
 +
<span style="font-family:Verdana; font-weight:700;"> 06/25/2011 </span>
 +
<br />
 +
1. Transform for 4 ligated genes: OLE1, MSN2, TSP1, ELO1
 +
Notes: use 5 µL  of samples
 +
<br />
 +
2. Inoculate for:
 +
BBa-K191005
 +
<br />
 +
BBa-K191004
 +
<br />
 +
BBa-K426020
 +
<br />
 +
BBa-I15010
 +
<br />

Latest revision as of 03:56, 29 September 2011

USC Banner.jpg


Laboratory Notebook

Brainstorming
Brainstorming

Week 1
Week 1

Week 2
Week 2

Week 3
Week 3

Week 4
Week 4

Week 5
Week 5

Week 6
Week 6

Week 7
Week 7

Week 8
Week 8

Week 9
Week 9

Week 10
Week 10

Week 11
Week 11

Week 12
Week 12

Week 13
Week 13

Week 14
Week 14

Week 3:

06/20/2011
1. Lab meeting
2. Ligate the blunted ended DNA


06/21/2011
1. Column purify the DNA
2. Make buffers for competent cells
Buffer: 10mM KOAc+80mM CaCl2-2H2O+70mM MnCl2-4H2O+10mM MgCl2-6H2O
3. Transformation again for:
Plasmids:OLE1, MSN2, TSP1, ELO1, CAS0, pSB1AK8
Vectors: prs 423-426


06/22/2011
1. Inoculation for :
pRS vectors 423-426 and pSB1AK8
2. PCR ligation products:
OLE1, MSN2, TSP1, ELO1


06/23/2011
1. Transfer E.Coli from agar stabs from iGEM delivery
BBa-K191005
BBa-K191004
BBa-K426020
BBa-I15010
Notes:
+ The agar should already have a hole from when it was stabbed. With an inoculating loop dipped into the stab, you can plate directly
onto a petri dish with the appropriate antibiotic;
+ Incubate the dish overnight at 37 C (14-16hrs)
+Pick a single colony to start up a culture
2. Gel running to test OLE1, MSN2, TSP1, ELO1, CAS0,
Observation: OLE was the only one with a clear band


06/24/2011
1. Observations of the E.Coli transformation from yesterday:
BBa-K191005 and BBa-K191004 are purple
BBa-I15010 is good


06/25/2011
1. Transform for 4 ligated genes: OLE1, MSN2, TSP1, ELO1 Notes: use 5 µL of samples
2. Inoculate for: BBa-K191005
BBa-K191004
BBa-K426020
BBa-I15010

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