Team:ITESM Mexico/Brainstorm

From 2011.igem.org

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<html><h1 class="myowntitle">Brainstorm</h1> </html>
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<h1>Know the process we follow for our project. Make click on the phase you want to know.</h1>
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<h1>Road to iGEM</h1>
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<h2>Know the process we followed to design our project. Click on the phase you want to know.</h2>
   
   
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         <li id="b1">1<img src="http://deviatan.com/sponsoring/brainstorm/brainstorm1.png" alt="Detection  of  salmonella  in food  samples" ></li>
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         <li id="b1">1<img src="http://deviatan.com/sponsoring/brainstorm/brainstorm1.png" alt="" ></li>
         <li id="b2">2<img src="http://deviatan.com/sponsoring/brainstorm/brainstorm2.png" alt="" ></li>
         <li id="b2">2<img src="http://deviatan.com/sponsoring/brainstorm/brainstorm2.png" alt="" ></li>
         <li id="b3">3<img src="http://deviatan.com/sponsoring/brainstorm/brainstorm3.png" alt="" ></li>
         <li id="b3">3<img src="http://deviatan.com/sponsoring/brainstorm/brainstorm3.png" alt="" ></li>
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        <center><h1 class="textcontent" id="title" ></h1></center>
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             Salmonella is one of the principal microorganisms that cause food poisoning. It is a major concern for the food industry as it contaminates a wide range of food products quite easily. The impact on public health is considerable because Salmonella can be found in every country, so it can be considered a global public health issue. Developing countries have trouble keeping the sanitary conditions to prevent salmonellosis and typhoid fever that is why an effective method of detection is needed.<br>
             Salmonella is one of the principal microorganisms that cause food poisoning. It is a major concern for the food industry as it contaminates a wide range of food products quite easily. The impact on public health is considerable because Salmonella can be found in every country, so it can be considered a global public health issue. Developing countries have trouble keeping the sanitary conditions to prevent salmonellosis and typhoid fever that is why an effective method of detection is needed.<br>
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                     <li>Identify the presence of xylene in liquid media using colorimetric techniques.</li>
                     <li>Identify the presence of xylene in liquid media using colorimetric techniques.</li>
                     <li>Construct a BioBrick to identify different concentrations of xylene using GFP.</li>
                     <li>Construct a BioBrick to identify different concentrations of xylene using GFP.</li>
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                     <li>Construct a novel mechanism using the BioBrick model that promotes the movement of E. coli on the medium following the concentration gradient of a contaminant as xylene. </li>
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                     <li>Construct a novel mechanism using the BioBrick model that promotes the movement of "E. coli" on the medium following the concentration gradient of a contaminant as xylene. </li>
                     <li>Determine the appropriate way to insert the constructed plasmid into Escherichia coli RP8611 CGSC.</li>
                     <li>Determine the appropriate way to insert the constructed plasmid into Escherichia coli RP8611 CGSC.</li>
                     <li>Determine the social, economic and ecological implications of identifying xylene as industrial residue.</li>
                     <li>Determine the social, economic and ecological implications of identifying xylene as industrial residue.</li>
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             The ability of expressing extracellular pili-like appendages called nanowires that enable electron transportation (cellular respiration) in adverse (electron acceptor deficient) conditions, is a shared characteristic among several microorganisms. Shewanella oneidensis MR-1 is capable of producing such nanowires when grown in low concentrations of O2 and metal ions. The genes mshA, pilA, and gspG have been targeted as the main nanowire coding genes, while omcA, mtrC, and gspD confer conductivity to the nanowires as they code for the cytochromes needed for electron transport. This project intends to transfer these mechanisms into a more convenient model organism, Escherichia coli to create a synthetic strain capable of expressing electrically conductive nanowires. Preliminary experiments suggest that E. coli HB101 does not present nanowires, so it is a suitable organism for recombination.
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             The ability of expressing extracellular pili-like appendages called nanowires that enable electron transportation (cellular respiration) in adverse (electron acceptor deficient) conditions, is a shared characteristic among several microorganisms. Shewanella oneidensis MR-1 is capable of producing such nanowires when grown in low concentrations of O2 and metal ions. The genes mshA, pilA, and gspG have been targeted as the main nanowire coding genes, while omcA, mtrC, and gspD confer conductivity to the nanowires as they code for the cytochromes needed for electron transport. This project intends to transfer these mechanisms into a more convenient model organism, Escherichia coli to create a synthetic strain capable of expressing electrically conductive nanowires. Preliminary experiments suggest that "E. coli" HB101 does not present nanowires, so it is a suitable organism for recombination.
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             Our goal is to create a recombinant E. coli strain, taking as a model E. coli HB101 and Shewanella oneidensis MR-1, capable of generating a difference of potential is the main objective of this project. In other words, we intend to integrate the electron conductivity mechanisms of S. oneidensis into E. coli.
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             Our goal is to create a recombinant "E. coli" strain, taking as a model "E. coli" HB101 and Shewanella oneidensis MR-1, capable of generating a difference of potential is the main objective of this project. In other words, we intend to integrate the electron conductivity mechanisms of S. oneidensis into "E. coli".
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b1: 'Detection  of  salmonella  in food  samples',
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b2: 'Intervention in extrinsic apoptosis pathways (Fas receptor) for the treatment of Systemic lupus erythematosus (SLE)',
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b3: 'Salmonella insullinae',
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b4: 'Aracnocoli',
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b5: 'CrazE. Coli Directed Motility Project. Aflatoxin detection',
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b6: 'CrazE. Coli Directed Motility Project. Xylene detection',
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b7: 'Genetically Modified Escherichia coli – An alternative way for electrical production in Microbial Fuel Cells'
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Latest revision as of 03:49, 29 September 2011

ITESM MÉXICO

SensE.coli

Igem Itesm


Brainstorm

Road to iGEM

Know the process we followed to design our project. Click on the phase you want to know.

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Retrieved from "http://2011.igem.org/Team:ITESM_Mexico/Brainstorm"