Road to iGEM
Know the process we followed to design our project. Click on the phase you want to know.
There are several methods for salmonella detection. Traditional methods such as the use of selective agar or standardized ISO methods can take up to 5 days. There are other rapid methods for detection and confirmation of Salmonella such as immunomagnetic separation, molecular methods like DNA hybridization and PCR assays, enzyme immunoassays (EIA), and enzyme-linked immunosorbent assays (ELISA). Some of these are claimed to give results within 48 hours.
The goal of this project is to develop a faster detection method using colorimetric techniques. Taking the base of the E. chromi project developed by the Cambridge 2009 iGEM team where there is no need to perform fluorescence microscopy.
A proposal is to develop a method to recombine Salmonella (if present) so that it produces a pigment visible to the human eye.
With the Systemic Lupus Erythematosus (SLE), the immunologic system will attack the healthy cells and tissues by mistake. This creates damage in the joints, skin, blood vessels and some organs. The SLE is one of the most common Lupus and it tends to affect a lot of parts of the body. The SLE is an autoimmune disease that causes the attack of the T cells to the healthy body cells, this causes the chronic inflammation.
The SLE can be mild o severe. Mild SLE can be controlled without many complications. Severe SLE can cause the death of the patient.
The cause of lupus has not been determined, but it's known that genes play an important role, although lupus is not determined just by them.
Our goal is to find an effective and specific treatment for systemic lupus erythematosus (SLE), minimizing secondary effects by attacking the reason of disease: over production of lymphocytes. This goal can be achieved by the altering of the immune dysregulation of SLE, interfering the pertinent apoptotic pathways. There are some genes, whose activation or deactivation, would nullify the problem of overproduced cells: Fas/Apo1/CD95.
The main objective of this proposal is to use designer bacteria to substitute the insulin injections to control glucose level in blood, by making the bacteria attach to the pancreas and then release the insulin when it is necessary. Diabetes type 1 is a chronic disease that occurs when the pancreas does not produce enough insulin, which is a hormone that regulates the blood sugar levels. Nowadays, more than 220 million people in the world has diabetes, and in 2005 approximately 1.1 million people died from diabetes. It is believed that diabetes deaths will double between 2005 and 2030. For that reasons, the project “Salmonella insullinae” would be a great option for the treatment of diabetes type I. The modeling of this project would consist in four phases: bacteria encapsulation, sensor, attachment, insulin production. Predicted results:
- Obtain a designer bacteria to substitute the insulin injections to control glucose level in blood, by making the bacteria attach to the pancreas and release the insulin when it is necessary
- Doing in silica experiments and if it´s possible we could do some experiments in vivo with mice we can probe that the Salmonella insulinae is a theoretical real option for diabetics.
- Spider dragline silkis five times stronger by weight than steel, three times tougher than the top quality man-made fiber Kevlar.
- To insert the gen MaSP1that codes for protein that is related with the synthesis of spider silk into an appropriate vector that could be BAC, plamid or cosmid. Make this technique to be standardized for further applications.
- To construct a system of biobricks that includes metabolic engineering.
- Silk protein expression in flask cultivation.
- Production of silk proteins by HCDC.
- Purification of recombinant silk protein.
- Fiber spinning and mechanical testing.
- The insertion of the gen in a vector, which is capable to support it and include the aspect of metabolic engineering; construction on biobricks.
- Identify the presence of xylene in liquid media using colorimetric techniques.
- Construct a BioBrick to identify different concentrations of xylene using GFP.
- Construct a novel mechanism using the BioBrick model that promotes the movement of "E. coli" on the medium following the concentration gradient of a contaminant as xylene.
- Determine the appropriate way to insert the constructed plasmid into Escherichia coli RP8611 CGSC.
- Determine the social, economic and ecological implications of identifying xylene as industrial residue.
- Increased movement speed by the over expression of flagellum and the addition of ATC to the medium.
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