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Team:USC/Notebook/Week1
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Week 1:
06/07/2011
Transformation plasmids into DH5α Competent cells
Plasmids are from iGEM plates:
A. Plate 2 Well 7E (AHL signaling)
B. Plate 3 Well 20F (LovTAP composite)
C. Plate 4 Well 16O (LovTAP Composite)
D. Plate 1 Well 2B (GFP+PEST191)
E. Plate 2 Well 4A (Yeast ADH1 promoter)
06/08/2011
Transformation of E. Coli, Plasmids are from iGEM plates:
Plasmids are from iGEM plates:
A. Plate 2 Well 13J
B. Plate 1 Well 20F
C. Plate 4 Well 16M
D. Plate 1 Well 16K
E. Plate 3 Well 14H
F. Plate 4 Well 6O
06/09/2011
1. Inoculation for all the plasmid we have transformed before.
Observation: all transformation except BBa-I15010
06/10/2011
1. Freeze the inoculated culture
2. Observation: BBa-I15008 and BBa-I63009don’t have as much growth as others
06/11/2011
1. Purify plasmid DNA from the culture
