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Team:Arizona State/Notebook/September
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== Thursday, September 1 == | == Thursday, September 1 == | ||
| - | + | * Started to develop Listeria Innocua project component. | |
| + | :* Listeria CRISPR only uses cas9 tracr gene | ||
| + | :* Project requires: | ||
| + | ::* Cas9 | ||
| + | ::* Duet plasmid | ||
| + | ::* Listeria T7 RSR | ||
| + | ::* Ligate all together in biobrick | ||
| + | ::* Test with protospacer | ||
== Saturday, September 3 == | == Saturday, September 3 == | ||
| - | + | * 3 PCR reactions using LI as template | |
| - | + | * Samples visualized on gel | |
| - | + | :* sample appears slightly higher than expected | |
| - | + | ::* some distortion and low resolution | |
| - | == | + | == Sunday, September 4 to Friday, September 9 == |
| - | + | * Cas9 PCR products restricted overnight | |
== Saturday, September 10 == | == Saturday, September 10 == | ||
| - | == Sunday, September 11 | + | * 9/10 restriction products dephosphorylated for 2 hours |
| - | + | * Overnight ligation of dephosphorylated restriction products | |
| - | + | == Sunday, September 11 to Saturday, September 17 == | |
| - | == | + | * 9/8 restriction - 9/10 ligation products transformed into NEB cells |
| - | + | == Sunday, September 18 to Saturday, September 24 == | |
| - | + | *Attempt to assemble CMR-RSR using overlapping oligos | |
| - | + | *Began preparation of pSB1C3 plasmid for BioBrick preparation | |
| - | == Sunday, September | + | == Sunday, September 25 to Wednesday, September 28 == |
| - | + | *Finalized BioBrick preparation for intended BioBrick part submission | |
| - | + | *Restrict, ligate, and transform CMR and pRSF. 9/25 Send sample to sequence. 9/27 Sequencing error. They will re-run. | |
| - | + | *Wiki parties all week in the lab! | |
| - | + | *Submitted BioBricks! | |
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Latest revision as of 03:09, 29 September 2011
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 Thursday, September 1
Saturday, September 3
Sunday, September 4 to Friday, September 9
Saturday, September 10
Sunday, September 11 to Saturday, September 17
Sunday, September 18 to Saturday, September 24
Sunday, September 25 to Wednesday, September 28
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