Team:Arizona State/Notebook/September

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Latest revision as of 03:09, 29 September 2011

Notebook: September

Thursday, September 1

Started to develop Listeria Innocua project component.

Listeria CRISPR only uses cas9 tracr gene
Project requires:

Cas9
Duet plasmid
Listeria T7 RSR
Ligate all together in biobrick
Test with protospacer

Saturday, September 3

3 PCR reactions using LI as template
Samples visualized on gel

sample appears slightly higher than expected

some distortion and low resolution

Sunday, September 4 to Friday, September 9

Cas9 PCR products restricted overnight

Saturday, September 10

9/10 restriction products dephosphorylated for 2 hours
Overnight ligation of dephosphorylated restriction products

Sunday, September 11 to Saturday, September 17

9/8 restriction - 9/10 ligation products transformed into NEB cells

Sunday, September 18 to Saturday, September 24

Attempt to assemble CMR-RSR using overlapping oligos
Began preparation of pSB1C3 plasmid for BioBrick preparation

Sunday, September 25 to Wednesday, September 28

Finalized BioBrick preparation for intended BioBrick part submission
Restrict, ligate, and transform CMR and pRSF. 9/25 Send sample to sequence. 9/27 Sequencing error. They will re-run.
Wiki parties all week in the lab!
Submitted BioBricks!

@@ Line 1: / Line 1: @@
 {{:Team:Arizona_State/Templates/main|title=Notebook: September|content=
+&nbsp;
 __NOTOC__
+== Thursday, September 1 ==
+* Started to develop Listeria Innocua project component.
+:* Listeria CRISPR only uses cas9 tracr gene
+:* Project requires:
+::* Cas9
+::* Duet plasmid
+::* Listeria T7 RSR
+::* Ligate all together in biobrick
+::* Test with protospacer
+== Saturday, September 3 ==
+* 3 PCR reactions using LI as template
+* Samples visualized on gel
+:* sample appears slightly higher than expected
+::* some distortion and low resolution
+== Sunday, September 4 to Friday, September 9 ==
+* Cas9 PCR products restricted overnight
+== Saturday, September 10 ==
+* 9/10 restriction products dephosphorylated for 2 hours
+* Overnight ligation of dephosphorylated restriction products
+== Sunday, September 11 to Saturday, September 17 ==
+* 9/8 restriction - 9/10 ligation products transformed into NEB cells
+== Sunday, September 18 to Saturday, September 24 ==
+*Attempt to assemble CMR-RSR using overlapping oligos
+*Began preparation of pSB1C3 plasmid for BioBrick preparation
+== Sunday, September 25 to Wednesday, September 28 ==
+*Finalized BioBrick preparation for intended BioBrick part submission
+*Restrict, ligate, and transform CMR and pRSF. 9/25 Send sample to sequence. 9/27 Sequencing error. They will re-run.
+*Wiki parties all week in the lab!
+*Submitted BioBricks!
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