Team:Calgary/Notebook/Protocols/Process18

From 2011.igem.org

(Difference between revisions)
 
(One intermediate revision not shown)
Line 23: Line 23:
<li>Place culture tube in incubator at 37°C overnight shaking vigorously (250 RPM)</li>
<li>Place culture tube in incubator at 37°C overnight shaking vigorously (250 RPM)</li>
   </ol>
   </ol>
-
 
+
<br></br><br></br><br></br>
</html>}}
</html>}}

Latest revision as of 03:02, 29 September 2011


Overnight Cultures

Reagents and Materials (per culture prepared):

  • 10mL culture tube. Use 16mm x 160mm or 16mm x 125mm
  • 5 mL LB
  • 5 uL 1000X antibiotics
  • Single colonies on a plate (best not to start an over night from a glycerol stock)

Protocol

  1. Add 5mL sterile/autoclaved LB in a 10mL culture tube.
  2. Pipet 5μL of 1000X antibiotic into the LB.
  3. Select a single colony using a sterile toothpick or pipette tip.
  4. Place toothpick or pipette tip in the culture tube and stir.
  5. Remove toothpick, or in the case of a pipette tip, leave in the tube.
  6. Place culture tube in incubator at 37°C overnight shaking vigorously (250 RPM)






Retrieved from "http://2011.igem.org/Team:Calgary/Notebook/Protocols/Process18"