Team:UTP-Panama/Week 17
From 2011.igem.org
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==September 27== | ==September 27== | ||
===WET LAB=== | ===WET LAB=== | ||
- | + | Transfer of a colony of RENBO into Liquid LB to grow overnight. | |
- | + | Preparation of Minimal Media and Saline Solutions for the experiment. | |
+ | We set up the experience designed in a total of 27 15mL Falcon tubes. | ||
==September 28== | ==September 28== |
Revision as of 02:28, 29 September 2011
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Week 17: September 26 to 28September 26WET LABTransformation of competent cells with Bristol 2010 - BBa_K381001. Experiment DesignWe designed the experiments we are going to do with the three BioBricks used (Bristol 2010 - BBa_K381001, GaTech 2010 – BBa_K410000, UTP-Panama 2011 – BBa_K672000). These temperatures were selected so we could evaluate the response to cold shock in various ranges of our BioBricks. Since the lab equipments we had available couldn’t reach temperatures between 11-20°C this temperature was not used during the experience. Finally, the lab equipment allowed us to make the experiments in: 37°C, 23°C and 8°C.
We also chose 3 different Growth Media: September 27WET LABTransfer of a colony of RENBO into Liquid LB to grow overnight. Preparation of Minimal Media and Saline Solutions for the experiment. We set up the experience designed in a total of 27 15mL Falcon tubes. September 28WET LAB(Objetives or Title): --ESCRIBIR Y MEJORAR LAB-- |