Team:Calgary/Notebook/Protocols
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<p>Here is a list of the procedures we used this summer. Each one has a description and list of materials needed.</p> | <p>Here is a list of the procedures we used this summer. Each one has a description and list of materials needed.</p> | ||
<ul> | <ul> | ||
- | <li><a href="https://2011.igem.org/Team:Calgary/Notebook/Protocols/Process1">Plasmid Extraction (from <i>Pseudomonas spp.</i>)</a></li> | + | |
+ | <h2>Microalgae Protocols</h2> | ||
+ | <li><a href="https://2011.igem.org/Team:Calgary/Notebook/Protocols/Process5">Preparation of Algal f2 Media</a></li> | ||
+ | <li><a href="https://2011.igem.org/Team:Calgary/Notebook/Protocols/Process6">Glass Bead Algal transformation</a></li> | ||
+ | <li><a href="https://2011.igem.org/Team:Calgary/Notebook/Protocols/Process7">Chloroplast Isolation</a></li> | ||
+ | <li><a href="https://2011.igem.org/Team:Calgary/Notebook/Protocols/Process8">Nuclear DNA extraction from Algae</a></li> | ||
+ | |||
+ | <h2>Pseudomonas Biotinylation Techniques</h2> | ||
+ | <li><a href="https://2011.igem.org/Team:Calgary/Notebook/Protocols/Process1">Plasmid Extraction (from <i>Pseudomonas spp.</i>)</a></li> | ||
+ | <li><a href="https://2011.igem.org/Team:Calgary/Notebook/Protocols/Process22">Biotinylation/Immunoprecipitation of Naphthenic Acids</a></li> | ||
+ | |||
+ | <h2>General Protocols</h2> | ||
+ | |||
<li><a href="https://2011.igem.org/Team:Calgary/Notebook/Protocols/Process21">Plasmid Extraction (from <i>E. coli</i>)</a></li> | <li><a href="https://2011.igem.org/Team:Calgary/Notebook/Protocols/Process21">Plasmid Extraction (from <i>E. coli</i>)</a></li> | ||
<li><a href="https://2011.igem.org/Team:Calgary/Notebook/Protocols/Process2">RNA Extraction</a></li> | <li><a href="https://2011.igem.org/Team:Calgary/Notebook/Protocols/Process2">RNA Extraction</a></li> | ||
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<li><a href="https://2011.igem.org/Team:Calgary/Notebook/Protocols/Process4">Quantitative Real Time PCR</a></li> | <li><a href="https://2011.igem.org/Team:Calgary/Notebook/Protocols/Process4">Quantitative Real Time PCR</a></li> | ||
<li><a href="https://2011.igem.org/Team:Calgary/Notebook/Protocols/pBAD">Testing the pBAD-LacZ Construct</a></li> | <li><a href="https://2011.igem.org/Team:Calgary/Notebook/Protocols/pBAD">Testing the pBAD-LacZ Construct</a></li> | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
<li><a href="https://2011.igem.org/Team:Calgary/Notebook/Protocols/Process9">Taq PCR Protocol</a></li> | <li><a href="https://2011.igem.org/Team:Calgary/Notebook/Protocols/Process9">Taq PCR Protocol</a></li> | ||
<li><a href="https://2011.igem.org/Team:Calgary/Notebook/Protocols/Process10">PCR Purification</a></li> | <li><a href="https://2011.igem.org/Team:Calgary/Notebook/Protocols/Process10">PCR Purification</a></li> | ||
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<li><a href="https://2011.igem.org/Team:Calgary/Notebook/Protocols/Process19">Preparing chemically competent cells (<i>E. coli</i>)</a></li> | <li><a href="https://2011.igem.org/Team:Calgary/Notebook/Protocols/Process19">Preparing chemically competent cells (<i>E. coli</i>)</a></li> | ||
<li><a href="https://2011.igem.org/Team:Calgary/Notebook/Protocols/Process20">Preparing glycerol stocks (<i>E. coli</i>) | <li><a href="https://2011.igem.org/Team:Calgary/Notebook/Protocols/Process20">Preparing glycerol stocks (<i>E. coli</i>) | ||
- | + | ||
</ul> | </ul> | ||
</html> | </html> | ||
}} | }} |
Revision as of 06:06, 28 September 2011
Protocols
Here is a list of the procedures we used this summer. Each one has a description and list of materials needed.
- Preparation of Algal f2 Media
- Glass Bead Algal transformation
- Chloroplast Isolation
- Nuclear DNA extraction from Algae
- Plasmid Extraction (from Pseudomonas spp.)
- Biotinylation/Immunoprecipitation of Naphthenic Acids
- Plasmid Extraction (from E. coli)
- RNA Extraction
- cDNA Synthesis
- Quantitative Real Time PCR
- Testing the pBAD-LacZ Construct
- Taq PCR Protocol
- PCR Purification
- Gel Extraction
- Testing the LacI-LacZ I732901 gene
- Bacterial Transformation
- Rehydration of Registry DNA
- Construction Techniques
- Agarose Gel Electrophresis
- LB Agar Plates
- Overnight Cultures
- Preparing chemically competent cells (E. coli)
- Preparing glycerol stocks (E. coli)