Team:UT Dallas/week12

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Revision as of 00:53, 28 September 2011

Week 11

9/11/11	Incubation: ToxR-FGFR, ctx-CheZ*, ctx-CheZ, ToxR, FGFR, CheY, CheZ,

9/12/11	Glycerol Stock: ToxR-FGFR, ctx-CheZ, ctx-CheZ, ToxR, FGFR, CheY, CheZ, Test Digestion/Gel Electrophoresis: FGFR-ToxR, ctx-CheZ-RBS-GFP Full Digestion/Gel Electrophoresis: FGFR-ToxR, ctx-CheZ-RBS-GFP Transformation: ToxR'MalE + ctx-CheZ-RBS-GFP, ToxR'MalE/ZIP + ctx-CheZ-RBS-GFP, ToxR'MalE/Zip L267S + ctx-CheZ-RBS-GFP Plating: ToxR'MalE/ZIP + ctx-CheZ-RBS-GFP Ligation: scp, Pyear, TetR, ToxR-FGFR, ctx Incubation: CheZ (Chlora)

9/13/11	Transform of PyeaR-ToxR-FGFR, TetR-ToxR-FGFR, scp-ToxR-FGFR Full Digestion: ToxR-FGFR (at E/X as vector) Ligation: Vector-> ToxR-FGFR Insert-> ctx Incubation: CheY

9/14/11	Transformation: ctx-ToxR-FGFR Test Digestion: ToxR'MalE, ToxR'MalE/ZIP, ToxR'MalE/Zip L267S Incubation: scp-ToxR-FGFR, TetR-ToxR-FGFR, PyeaR-ToxR-FGFR (Carb) Miniprep: CheY PCR: CheY Incubation: ToxR'MalE, ToxR'MalE/ZIP, CheZ*

9/15/11	PCR: CheY Full Digestion: CheY (X/S), vector pSB1C3 Miniprep/Glycerol Stock: pYeaR-ToxR-FGFR, scp-ToxR-FGFR, ToxR'MalE, ToxR'MalE/ZIP Test Digestion: pYeaR-ToxR-FGFR, scp-ToxR-FGFR, ToxR'MalE, ToxR'MalE/ZIP Dephosphorylation: Vector pSB1C3 Ligation: CheY + pSB1c3 Full Digestion/Gel Elctrophoresis/Purification: pYeaR-ToxR-FGFR, scp-ToxR-FGFR, ToxR'MalE, ToxR'MalE/ZIP, CheZ, CheZ, ctx-CheZ-RBS-GFP, ctx Ligation: ctx + CheZ Transformation: pSB1C3 Vector, pYeaR-ToxR-FGFR, ctx-CheZ-RBS-GFP, CheZ, ctx-GFP, ctx, Chey

9/16/11	Digestion: ctx-CheZ Incubation: ToxR'MalE/ZIP, ToxR'MalE, Ligation: ctx + CheY (retry)

9/17/11	Incubation: pYeaR-ToxR-FGFR, ctx-CheZ*, CheY

@@ Line 1: / Line 1: @@
-<h2>Week 12</h2>
+<h2>Week 11</h2>
 <table>
-<tr><td valign='top'>
+<tr><td valign='top'>9/11/11</td><td valign='top'>Incubation: ToxR-FGFR, ctx-CheZ*, ctx-CheZ, ToxR, FGFR, CheY, CheZ,
-/18/11</td><td valign='top'> Miniprep/Glycerol Stock: pYeaR-ToxR-FGFR, ctx-CheZ*, CheY<br>
+</td></tr><tr><td valign='top'>&nbsp;</td><td valign='top'>&nbsp;</td></tr>
-	 Test Digestion: pYeaR-ToxR-FGFR, ctx-CheZ*, CheY <br>
+<tr><td valign='top'>9/12/11</td><td valign='top'>Glycerol Stock: ToxR-FGFR, ctx-CheZ*, ctx-CheZ, ToxR, FGFR, CheY, CheZ,<br>
-	 Colony PCR: pYeaR-ToxR-FGFR<br>
+	 Test Digestion/Gel Electrophoresis: FGFR-ToxR, ctx-CheZ-RBS-GFP<br>
-	 Test Digestion: CheY (X/S)
+	 Full Digestion/Gel Electrophoresis: FGFR-ToxR, ctx-CheZ-RBS-GFP<br>
-</td></tr><tr><td valign='top'>&nbsp;</td><td valign='top'>&nbsp;</td></tr><tr><td valign='top'>
+	 Transformation: ToxR'MalE + ctx-CheZ-RBS-GFP, ToxR'MalE/ZIP + ctx-CheZ-RBS-GFP, ToxR'MalE/Zip L267S + ctx-CheZ-RBS-GFP<br>
-/19/11</td><td valign='top'> Full Digestion/Electrophoresis: CheY, CheZ*, pSB1C3<br>
+	 Plating: ToxR'MalE/ZIP + ctx-CheZ-RBS-GFP<br>
-	 PCR/Gel Electrophoresis: CheY<br>
+	 Ligation: scp, Pyear, TetR, ToxR-FGFR, ctx<br>
-	 Ligation: ctx + CheZ*, ctx + CheY, pYeaR-ToxR-FGFR + pSB1C3, ctx-CheZ-RBS-GFP + pSB1C3<br>
+	 Incubation: CheZ* (Chlora)
-	 Incubation: scp-ToxR-FGFR, ToxR'MalE/ZIP, ToxR'MalE
+</td></tr><tr><td valign='top'>&nbsp;</td><td valign='top'>&nbsp;</td></tr>
-</td></tr><tr><td valign='top'>&nbsp;</td><td valign='top'>&nbsp;</td></tr><tr><td valign='top'>
+<tr><td valign='top'>9/13/11</td><td valign='top'>Transform of PyeaR-ToxR-FGFR, TetR-ToxR-FGFR, scp-ToxR-FGFR<br>
-/20/11</td><td valign='top'> PCR/Gel Purify: ctx<br>
+	 Full Digestion: ToxR-FGFR (at E/X as vector)<br>
-	 Maltose Induction Experiment: DATA COLLECTION<br>
+	 Ligation: Vector-> ToxR-FGFR   Insert-> ctx<br>
-	 Miniprep: scp-ToxR-FGFR<br>
+	 Incubation: CheY
-	 Full Digestion/Gel Purification: CheZ*, CheY<br>
+</td></tr><tr><td valign='top'>&nbsp;</td><td valign='top'>&nbsp;</td></tr>
-	 Ligation: ctx + CheZ*, ctx + CheY, ctx + CheZ-RBS-GFP<br>
+<tr><td valign='top'>9/14/11</td><td valign='top'>Transformation: ctx-ToxR-FGFR<br>
-	 Transformation: ToxR'MalE + ctx-GFP, ToxR'MalE/ZIP + ctx-GFP, ToxR'MalE/ZIP L267S + ctx-GFP<br>
+	 Test Digestion: ToxR'MalE, ToxR'MalE/ZIP, ToxR'MalE/Zip L267S<br>
-	 Icubation: pYeaR-ToxR-FGFR (Chlora)<br>
+	 Incubation: scp-ToxR-FGFR, TetR-ToxR-FGFR, PyeaR-ToxR-FGFR (Carb)<br>
-	 Miniprep/Glycerol Stock:  pYeaR-ToxR-FGFR
+	 Miniprep: CheY<br>
-</td></tr><tr><td valign='top'>&nbsp;</td><td valign='top'>&nbsp;</td></tr><tr><td valign='top'>
+	 PCR: CheY<br>
-/21/11</td><td valign='top'> PCR: ctx (retry)<br>
+	 Incubation: ToxR'MalE, ToxR'MalE/ZIP, CheZ*
-	 Test Digestion/Purification: pYeaR-ToxR-FGFR<br>
+</td></tr><tr><td valign='top'>&nbsp;</td><td valign='top'>&nbsp;</td></tr>
-	 Full Digestion/PurificationL pYeaR-ToxR-FGFR<br>
+<tr><td valign='top'>9/15/11</td><td valign='top'>PCR: CheY<br>
-	 Dephosphorylation: pSB1C3<br>
+	 Full Digestion: CheY (X/S), vector pSB1C3<br>
-	 Incubation: ctx-gfp, BL21 E. coli<br>
+	 Miniprep/Glycerol Stock: pYeaR-ToxR-FGFR, scp-ToxR-FGFR, ToxR'MalE, ToxR'MalE/ZIP<br>
-	 Transformation: Germany Parts (FHK12)<br>
+	 Test Digestion: pYeaR-ToxR-FGFR, scp-ToxR-FGFR, ToxR'MalE, ToxR'MalE/ZIP<br>
-	 Gel Purification: ctx<br>
+	 Dephosphorylation: Vector pSB1C3<br>
-	 Ligation: ctx + CheY<br>
+	 Ligation: CheY + pSB1c3<br>
-	 Incubation: ctx-CheZ*
+	 Full Digestion/Gel Elctrophoresis/Purification: pYeaR-ToxR-FGFR, scp-ToxR-FGFR, ToxR'MalE, ToxR'MalE/ZIP, CheZ, CheZ*, ctx-CheZ-RBS-GFP, ctx<br>
-</td></tr><tr><td valign='top'>&nbsp;</td><td valign='top'>&nbsp;</td></tr><tr><td valign='top'>
+	 Ligation: ctx + CheZ*<br>
-/22/11</td><td valign='top'> Miniprep/Glycerol Stock: ctx-GFP, ctx-CheZ, ctx-CheY<br>
+	 Transformation: pSB1C3 Vector, pYeaR-ToxR-FGFR, ctx-CheZ-RBS-GFP, CheZ, ctx-GFP, ctx, Chey
-	 Full Digestion: pSB1C3<br>
+</td></tr><tr><td valign='top'>&nbsp;</td><td valign='top'>&nbsp;</td></tr>
-	 Make BL21 competent<br>
+<tr><td valign='top'>9/16/11</td><td valign='top'>Digestion: ctx-CheZ<br>
-	 Dephosphorylation: pSB1C3<br>
+	 Incubation: ToxR'MalE/ZIP, ToxR'MalE, <br>
-	 Ligation: ctx + CheY, ctx + FGFR-ToxR<br>
+	 Ligation: ctx + CheY (retry)<br>
-	 Transformation: (BL21) pcst-RBS-LuxI, AHL inducible colicin, ctx-CheY, ctx-ToxR-FGFR<br>
+</td></tr><tr><td valign='top'>&nbsp;</td><td valign='top'>&nbsp;</td></tr>
-	 Ligation: scp-ToxR-GFP + pSB1C3, ctx-CheZ-RBS-GFP + pSB1C3, ctx-Chez + pSB1C3<br>
+<tr><td valign='top'>9/17/11</td><td valign='top'>Incubation: pYeaR-ToxR-FGFR, ctx-CheZ*, CheY</td></tr>
-	 Incubation: psct-RBS-LuxI-term
-</td></tr><tr><td valign='top'>&nbsp;</td><td valign='top'>&nbsp;</td></tr><tr><td valign='top'>
-/23/11</td><td valign='top'> Miniprep: psct-RBS-LuxI-term<br>
-	 Dissolving/Diluting Primers: Germany Parts<br>
-:20 Dilution of scp-ToxR-FGFR + ctx-GFP, pYeaR-ToxR-FGFR + ctx-GFP <br>
-	 Glycerol Stock: scp-ToxR-FGFR + ctx-GFP, pYeaR-ToxR-FGFR + ctx-GFP <br>
-	 Transformation: German Parts<br>
-	 Incubation: scp-ToxR-FGFR, pYeaR-ToxR-FGFR
-</td></tr><tr><td valign='top'>&nbsp;</td><td valign='top'>&nbsp;</td></tr><tr><td valign='top'>
-/24/11</td><td valign='top'> Dilution 1:10 for Optical Density readings: scp-ToxR-FGFR, pYeaR-ToxR-FGFR<br>
-	 Growing from glycerol stock (backup): scp-ToxR-FGFR, pYeaR-ToxR-FGFR<br>
-	 PCR: German Parts<br>
-	 Digestion: pSB1C3 (X/S)  <br>
-	 Heparin/FGF Induction Experiment: DATA COLLECTION (Fluorescence)<br>
-	 Dephosphorylation: Germany Parts, pSB1C3<br>
-	 Ligation: <br> &nbsp;&nbsp;&nbsp;&nbsp;ctx-CheZ + pSB1C3, scp-ToxR-FGFR + pSB1C3, ctx-CheZ-RBS-GFP + pSB1C3, pSB1C3-CheY + ctx, pSB1C3-CheZ* + ctx, pSb1C3-ToxR-FGFR + ctx<br>
-	 Transformation: <br>&nbsp;&nbsp;&nbsp;&nbsp;ctx-CheZ + pSB1C3, scp-ToxR-FGFR + pSB1C3, ctx-CheZ-RBS-GFP + pSB1C3, pSB1C3-CheY + ctx, pSB1C3-CheZ* + ctx, pSb1C3-ToxR-FGFR + ctx
-	 Transformation: <br>&nbsp;&nbsp;&nbsp;&nbsp;ToxR'MalE, ToxR'MalE/ZIP, ToxR'MalE/ZIP L267S</td></tr>
-<tr><td valign='top'>
-/25/11</td><td valign='top'> Dilution 1:20 for experimentation: ToxR'MalE, ToxR'MalE/ZIP, ToxR'MalE/ZIP L267S <br>
-	 Running Maltose Induction Experiment: DATA COLLECTION (fluorescence)	  <br>
-	 Incubation: scp-ToxR-FGFR, pYeaR-ToxR-FGFR, 	 <br>
-	 Incubation of fluorescent parts: scp-ToxR-FGFR, pYeaR-ToxR-FGFR,
-</td></tr><tr><td valign='top'>&nbsp;</td><td valign='top'>&nbsp;</td></tr><tr><td valign='top'>
-/26/11</td><td valign='top'> Dilution 1:5 for experimentation: pYear-ToxR-FGFR, scp-ToxR-FGFR	  <br>
-	 Heparin/FGF Induction Experiment (re-run): DATA COLLECTION (fluorescence)    <br>
-	 Incubation (for iGem Registry): FGFR, CheZ*, ToxR-FGFR</td></tr>
 </table>