Team:Minnesota/leex4462-04162011000200-28

(Difference between revisions)

Latest revision as of 22:47, 27 September 2011

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Project: Surface Display

Date: Sat, 16 Apr 2011 00:02:00 GMT

Author: Sukdong Lee (leex4462)

Access: Public

Revision History:

Mon, 22 Aug 2011 10:00:33 GMT (leex4462): entry created in project'Surface Display' by leex4462 (id=3)

Purpose:

Redo the digest of both insert and vector.

Procedure

-Use NdeI and NotI with NEB Buffer 3.

-Add 0.4 uL of BSA to prevent degradation or star activity (most likely due to NdeI).

-Check the products after 1-2 hours to see if degradation occurred.


240px

@@ Line 1: / Line 1: @@
 __NOTOC__
 {{Team:Minnesota/Top}}
-<br>== Restriction Digest of pucBB and ompA inserts ==
+<br>
+== Restriction Digest of pucBB and ompA inserts ==
 '''Project:''' Surface Display
@@ Line 28: / Line 29: @@
 <nowiki>-Check the products after 1-2 hours to see if degradation occurred.</nowiki>
 {{Team:Minnesota/Bottom}}