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Team:UNICAMP-EMSE Brazil/Results
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The students assembled the devices subparts: the SoxR/SoxS sensor ([https://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Project#Device_2:_NO_sensor.2FIL-10_producer Device 2, NO sensor device/ IL-10 producer]), the Adrenaline sensor ([https://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Project#Device_1:_Adrenaline_sensor.2FIL-12_producer Device 1, CA/AI-3 sensor device/ IL-12 producer]), and the hemolysin secretion system ([https://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Project#Device_3:_Secretion_system Device 3, Protein Secretion System]). All the sensors were also built with GFP downstream of the sensor itself, replacing the Device products: IL-12 at [https://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Project#Device_1:_Adrenaline_sensor.2FIL-12_producer Device 1] and IL-10 at [https://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Project#Device_2:_NO_sensor.2FIL-10_producer Device 2]. | The students assembled the devices subparts: the SoxR/SoxS sensor ([https://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Project#Device_2:_NO_sensor.2FIL-10_producer Device 2, NO sensor device/ IL-10 producer]), the Adrenaline sensor ([https://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Project#Device_1:_Adrenaline_sensor.2FIL-12_producer Device 1, CA/AI-3 sensor device/ IL-12 producer]), and the hemolysin secretion system ([https://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Project#Device_3:_Secretion_system Device 3, Protein Secretion System]). All the sensors were also built with GFP downstream of the sensor itself, replacing the Device products: IL-12 at [https://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Project#Device_1:_Adrenaline_sensor.2FIL-12_producer Device 1] and IL-10 at [https://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Project#Device_2:_NO_sensor.2FIL-10_producer Device 2]. | ||
| + | ==Device 2 testing: SoxR/SoxS system regulating GFP production== | ||
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| + | The [https://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Project#Device_2:_NO_sensor.2FIL-10_producer Device 2] is a modification of Stanford team anti-inflammatory device (iGEM 2009 - https://2009.igem.org/Team:Stanford/ProjectPage), which comprises SoxR gene (BBa_K223047) under control of a Constitutive Promoter (BBa_J23119) and SoxS promoter (BBa_K223001 – deleted part), coupled to human IL-10 gene (sequence designed by the team, improved for bacterial expression). | ||
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| + | In order to test the ability of Jedi Bacteria in sensing NO levels and activating genes linked to SoxS promoter, we built a Device testing, with GFP linked to SoxS promoter, as it is shown in the following schema (Figure 1): | ||
[[Image:UNICAMP_EMSE_GFP_SOX_device.jpg|center|700px]] | [[Image:UNICAMP_EMSE_GFP_SOX_device.jpg|center|700px]] | ||
| + | '''Figure 1: Testing [https://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Project#Device_2:_NO_sensor.2FIL-10_producer Device 2] through replacement of IL-10 to GFP. | ||
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| + | The ability to recognize NO (nitric oxide), an inflammation signal molecule, was characterized for SoxR/SoxS sensor, and <font color=red>'''found to be FUNCTIONAL'''. In the section below we present the detailed results. | ||
Revision as of 03:36, 27 September 2011
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Overview
Functional tests
The students assembled the devices subparts: the SoxR/SoxS sensor (Device 2, NO sensor device/ IL-10 producer), the Adrenaline sensor (Device 1, CA/AI-3 sensor device/ IL-12 producer), and the hemolysin secretion system (Device 3, Protein Secretion System). All the sensors were also built with GFP downstream of the sensor itself, replacing the Device products: IL-12 at Device 1 and IL-10 at Device 2.
Device 2 testing: SoxR/SoxS system regulating GFP production
The Device 2 is a modification of Stanford team anti-inflammatory device (iGEM 2009 - https://2009.igem.org/Team:Stanford/ProjectPage), which comprises SoxR gene (BBa_K223047) under control of a Constitutive Promoter (BBa_J23119) and SoxS promoter (BBa_K223001 – deleted part), coupled to human IL-10 gene (sequence designed by the team, improved for bacterial expression).
In order to test the ability of Jedi Bacteria in sensing NO levels and activating genes linked to SoxS promoter, we built a Device testing, with GFP linked to SoxS promoter, as it is shown in the following schema (Figure 1):
Figure 1: Testing Device 2 through replacement of IL-10 to GFP.
The ability to recognize NO (nitric oxide), an inflammation signal molecule, was characterized for SoxR/SoxS sensor, and found to be FUNCTIONAL. In the section below we present the detailed results.
