Team:Caltech/Notebook

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Latest revision as of 20:28, 26 September 2011

Caltech iGEM 2011

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Methods and Protocols

Week 1: 6/15-6/17: Lab orientation

Week 2: 6/19-6/25: Lab training; sample collection, enrichment cultures, DNA extraction from samples

Week 3: 6/26-7/2: Prepare parts by PCR for Gibson assembly of test plasmids of BisdA and BisdB, continue enrichment cultures

Week 4: 7/3-7/10: Sequencing and testing of Gibson assembled vectors

Week 5: 7/11-7/16: Remake Gibson vectors, begin 16S sequencing, run pulsed field gel to extract degradation genes for fosmid kit

Week 6: 7/17-7/23: Continuation of Gibson assembly, 16s sequencing and assembly, and fosmid kit

Week 7: 7/24-7/30: p450 testing, plating of EDC's, more assembly

Week 8: 7/31-8/6: Titering of fosmid kits, HPLC of p450 degradation, more assembly and EDC plating

Week 9: 8/7-8/13 GCMS attempts at p450-degraded BPA, biofilm/crystal violet experiments, assembly of constructs and DDT gene

Week 10: 8/14-8/20: Electrospray of p450-degraded BPA; visits to local water treatment plants; assembly of constructs, lacZ, and DDT gene

Week 11: 8/21-8/27: Assembly of DDT-degradation gene and p450 into BioBrick submission and pET protein expression vectors

Week 12: 8/28-9/3: Biofilm-X-gal experiments, sequencing, HPLC

Week 13: 9/4-9/10: Quikchange, Biobrick, sequencing

Week 14: 9/12-9/18: Redo Quikchange, Biofilm, Protein Expression, HPLC

Week 15: 9/19-9/23: Submit parts to registry: DDT gene, bisdA & bisdB, Wt-F87A

@@ Line 8: / Line 8: @@
 <p>[[Team:Caltech/Week_1|Week 1]]: 6/15-6/17: Lab orientation</p>
 <p>[[Team:Caltech/Week_2|Week 2]]: 6/19-6/25: Lab training; sample collection, enrichment cultures, DNA extraction from samples</p>
-<p>[[Team:Caltech/Week_3|Week 3]]: 6/26-7/2:
+<p>[[Team:Caltech/Week_3|Week 3]]: 6/26-7/2: Prepare parts by PCR for Gibson assembly of test plasmids of BisdA and BisdB, continue enrichment cultures</p>
+<p>[[Team:Caltech/Week_4|Week 4]]: 7/3-7/10: Sequencing and testing of Gibson assembled vectors</p>
-You should make use of the calendar feature on the wiki and start a lab notebook.  This may be looked at by the judges to see how your work progressed throughout the summer.  It is a very useful organizational tool as well.
+<p>[[Team:Caltech/Week_5|Week 5]]: 7/11-7/16: Remake Gibson vectors, begin 16S sequencing, run pulsed field gel to extract degradation genes for fosmid kit</p>
+<p>[[Team:Caltech/Week_6|Week 6]]: 7/17-7/23: Continuation of Gibson assembly, 16s sequencing and assembly, and fosmid kit</p>
+<p>[[Team:Caltech/Week_7|Week 7]]: 7/24-7/30: p450 testing, plating of EDC's, more assembly</p>
+<p>[[Team:Caltech/Week_8|Week 8]]: 7/31-8/6: Titering of fosmid kits, HPLC of p450 degradation, more assembly and EDC plating</p>
+<p>[[Team:Caltech/Week_9|Week 9]]: 8/7-8/13 GCMS attempts at p450-degraded BPA, biofilm/crystal violet experiments, assembly of constructs and DDT gene</p>
+<p>[[Team:Caltech/Week_10|Week 10]]: 8/14-8/20: Electrospray of p450-degraded BPA; visits to local water treatment plants; assembly of constructs, lacZ, and DDT gene</p>
+<p>[[Team:Caltech/Week_11|Week 11]]: 8/21-8/27: Assembly of DDT-degradation gene and p450 into BioBrick submission and pET protein expression vectors</p>
+<p>[[Team:Caltech/Week_12|Week 12]]: 8/28-9/3: Biofilm-X-gal experiments, sequencing, HPLC</p>
+<p>[[Team:Caltech/Week_13|Week 13]]: 9/4-9/10: Quikchange, Biobrick, sequencing</p>
+<p>[[Team:Caltech/Week_14|Week 14]]: 9/12-9/18: Redo Quikchange, Biofilm, Protein Expression, HPLC</p>
+<p>[[Team:Caltech/Week_15|Week 15]]: 9/19-9/23: Submit parts to registry: DDT gene, bisdA & bisdB, Wt-F87A</P>
+<!-- You should make use of the calendar feature on the wiki and start a lab notebook.  This may be looked at by the judges to see how your work progressed throughout the summer.  It is a very useful organizational tool as well. -->
 }}