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Notebook - June 2011

June 29, 2011

We did a lot of work today like:

1. The miniprep of the strains containing the BioBrick rh1AB.

2. An electrophoresis in agarose gel at 1.5% in wich we observed the plasmidic DNA.

3. Mean while some of us picked colonies with P+RBS and the GFP+Ter and transferred it to LB medium.

4. Last attempt in seed the E. coli containing the rh1AB mutated from 2/3/11 in LB medium but it didn´t grow again! =(. This time we putted the exact concentration of cloranfenicol (33 ug/ml).

5. We did all the necessary calculations for the antibiotics concentrations.

Rh1AB + psB1C3 Ectroforesis

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1 and 8: Marker 100- 3000 pb. 2-7: rh1AB 2010 BioBrick