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Team:Panama/Notebook/June2011
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| + | =='''June 29, 2011'''== | ||
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| + | We did a lot of work today like: | ||
| + | 1. The miniprep of the strains containing the BioBrick rh1AB. | ||
| + | 2. An electrophoresis in agarose gel at 1.5% in wich we observed the plasmidic DNA. | ||
| + | 3. Mean while some of us picked colonies with P+RBS and the GFP+Ter and transferred it to LB medium. | ||
| + | 4. Last attempt in seed the E. coli containing the rh1AB mutated from 2/3/11 in LB medium but it didn´t grow again! =(. This time we putted the exact concentration of cloranfenicol (33 ug/ml). | ||
| + | 5. We did all the necessary calculations for the antibiotics concentrations. | ||
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| + | '''Rh1AB + psB1C3 Ectroforesis''' | ||
| + | [[File:e29-7-11.jpg]] | ||
Revision as of 16:34, 26 September 2011
Notebook - June 2011
June 29, 2011
We did a lot of work today like: 1. The miniprep of the strains containing the BioBrick rh1AB. 2. An electrophoresis in agarose gel at 1.5% in wich we observed the plasmidic DNA. 3. Mean while some of us picked colonies with P+RBS and the GFP+Ter and transferred it to LB medium. 4. Last attempt in seed the E. coli containing the rh1AB mutated from 2/3/11 in LB medium but it didn´t grow again! =(. This time we putted the exact concentration of cloranfenicol (33 ug/ml). 5. We did all the necessary calculations for the antibiotics concentrations.
Rh1AB + psB1C3 Ectroforesis
File:E29-7-11.jpg
