Team:Harvard/Results/Zinc Finger Binders

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(Color Blindness (CB) Bottom Hits)
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[[Team:Harvard/Results | Overview]] | [[Team:Harvard/Results/MAGE | MAGE]] | [[Team:Harvard/Results/Lambda_Red | Lambda Red]]| [[Team:Harvard/Results/Chip_Synthesis | Chip-Based Library]] | [[Team:Harvard/Results/One-Hybrid_Selection | One-Hybrid Selection]] | [[Team:Harvard/Results/Zinc_Finger_Binders | Zinc Finger Binders]] | [[Team:Harvard/Results/Biobricks | Biobricks]]
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Revision as of 00:43, 26 September 2011

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Color Blindness (CB) Bottom Hits

We assembled the Color Blindness Bottom library, transformed it into the proper selection strain, and plated on incomplete media with varying concentrations of 3-AT. We saw colonies form, with more on the less stringent plates (NM) and fewer colonies on the higher 3-AT concentrations. Several colonies were picked and tested under varying concentrations of 3-AT in plate reader overnight, and there was a clear difference in growth between the colonies and the negative control (CB bottom binding site with Zif268). After sequencing, we identified xx novel zinc fingers!

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