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Team:UT Dallas/ligation
From 2011.igem.org
(Difference between revisions)
| Line 178: | Line 178: | ||
<tr> | <tr> | ||
<td>Total Reaction Mix(µl)</td> | <td>Total Reaction Mix(µl)</td> | ||
| - | <td><input type='text' id='total_vol' value='10'></td> | + | <td><input type='text' id='total_vol' value='10' onkeyup="javascript:doCalculate()"></td> |
<td> </td> | <td> </td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>Volume of the buffer(µl)</td> | <td>Volume of the buffer(µl)</td> | ||
| - | <td><input type='text' id='buffer_vol' value='1'></td> | + | <td><input type='text' id='buffer_vol' value='1' onkeyup="javascript:doCalculate()"></td> |
<td> </td> | <td> </td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>Volume of the enzyme(µl)</td> | <td>Volume of the enzyme(µl)</td> | ||
| - | <td><input type='text' id='enzyme_vol' value='1'></td> | + | <td><input type='text' id='enzyme_vol' value='1' onkeyup="javascript:doCalculate()"></td> |
<td> </td> | <td> </td> | ||
</tr> | </tr> | ||
| Line 203: | Line 203: | ||
<tr> | <tr> | ||
<td>Molar Ratio</td> | <td>Molar Ratio</td> | ||
| - | <td><input type='text' id='Molar_Ratio_V' value=' | + | <td><input type='text' id='Molar_Ratio_V' value='1' onkeyup="javascript:doCalculate()"></td> |
| - | <td><input type='text' id='Molar_Ratio_I' value=' | + | <td><input type='text' id='Molar_Ratio_I' value='3' onkeyup="javascript:doCalculate()"></td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>Concentration(ng/µl)</td> | <td>Concentration(ng/µl)</td> | ||
| - | <td><input type='text' id='Conc_V' value=' | + | <td><input type='text' id='Conc_V' value='1000' onkeyup="javascript:doCalculate()"></td> |
| - | <td><input type='text' id='Conc_I' value=' | + | <td><input type='text' id='Conc_I' value='50' onkeyup="javascript:doCalculate()"></td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>Length(bp)</td> | <td>Length(bp)</td> | ||
| - | <td><input type='text' id='Length_V' value=' | + | <td><input type='text' id='Length_V' value='3000' onkeyup="javascript:doCalculate()"></td> |
| - | <td><input type='text' id='Length_I' value=' | + | <td><input type='text' id='Length_I' value='150' onkeyup="javascript:doCalculate()"></td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>Maximum volume(µl)</td> | <td>Maximum volume(µl)</td> | ||
| - | <td><input type='text' id='max_vol_V' value=' | + | <td><input type='text' id='max_vol_V' value='5' onkeyup="javascript:doCalculate()"></td> |
| - | <td><input type='text' id='max_vol_I' value=' | + | <td><input type='text' id='max_vol_I' value='5' onkeyup="javascript:doCalculate()"></td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
Revision as of 14:08, 25 September 2011
Introduction >> Ligation Calculator
2011 iGEM Main Page
| Total Reaction Mix(µl) | ||
| Volume of the buffer(µl) | ||
| Volume of the enzyme(µl) | ||
| Vector | Insert | |
| Molar Ratio | ||
| Concentration(ng/µl) | ||
| Length(bp) | ||
| Maximum volume(µl) | ||
| Known Vector Lengths | ||
Ligation Mix
| Vol. in µl | |
| Buffer | 1 |
| Enzyme | 1 |
| Vector | 0 |
| Insert | 0 |
| Water | 0 |
| Total | 10 |
