Team:UC Davis/Data LacI

From 2011.igem.org

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{{Team:UC_Davis/Head}}
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{{Team:UC_Davis/MutantWidget}}
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Some preliminary data.  These are LacI promoter mutants.  The green bars represent mutants that are outside of 1.5 standard deviations of wildtype LacI promoter.  There's still more to come!
Some preliminary data.  These are LacI promoter mutants.  The green bars represent mutants that are outside of 1.5 standard deviations of wildtype LacI promoter.  There's still more to come!
</center>
</center>
 +
 +
<div id="mutantwidget" class="floatbox2">
 +
<h3>Wildtype</h3>
 +
<span class="sequence">caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca</span>
 +
<span class="xdata">0, .0014, .0028, .0042, .0056, .0070, .0140</span>
 +
<span class="ydata">
 +
0.99315,
 +
  0.78019, 
 +
  0.58405,
 +
  0.45400, 
 +
  0.35401,
 +
  0.29099, 
 +
  0.13305, 
 +
</span>
 +
<span class="numconditions">4</span>
 +
<span class='xdata_3d'>0, 1, 2, 5</span>
 +
<span class='ydata_3d'>0.00000,  0.20000,  0.40000,  0.60000,  0.80000,  1.00000,  2.00000</span>
 +
<span class='zdata_3d'>
 +
  0.99315,  1.02097,  0.99023,  0.99354,
 +
  0.78019,  0.97021,  0.96725,  0.96226,
 +
  0.58405,  0.87100,  0.89795,  0.94529,
 +
  0.45400,  0.76333,  0.85071,  0.93618,
 +
  0.35401,  0.65243,  0.75444,  0.88056,
 +
  0.29099,  0.55454,  0.68266,  0.84441,
 +
  0.13305,  0.26829,  0.37015,  0.55294</span>
 +
<h3>Mutant 1</h3>
 +
<span class="sequence">caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca</span>
 +
<span class="xdata">0, .0014, .0028, .0042, .0056, .0070, .0140</span>
 +
<span class="ydata">
 +
  0.68829,
 +
  0.48655, 
 +
  0.27198,
 +
  0.15260, 
 +
  0.08426,
 +
  0.05322, 
 +
  0.01741, 
 +
</span>
 +
<h3>Mutant 2</h3>
 +
<span class="sequence">caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattattgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacgca</span>
 +
<span class="xdata">0, .0014, .0028, .0042, .0056, .0070, .0140</span>
 +
<span class="ydata">
 +
  0.68514,
 +
  0.43727, 
 +
  0.27724,
 +
  0.17817, 
 +
  0.11684,
 +
  0.08021, 
 +
  0.03415, 
 +
</span>
 +
<h3>Mutant 3</h3>
 +
<span class="sequence">caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaaagtgagttagctcactcattaggcaccccaggctttacactttaagcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca</span>
 +
<span class="xdata">0, .0014, .0028, .0042, .0056, .0070, .0140</span>
 +
<span class="ydata">
 +
  0.67026,
 +
  0.44322, 
 +
  0.28504,
 +
  0.17543, 
 +
  0.10938,
 +
  0.07325, 
 +
  0.02937, 
 +
</span>
 +
<h3>Mutant 4</h3>
 +
<span class="sequence">caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgcgcgcaacgcaactaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacgca</span>
 +
<span class="xdata">0, .0014, .0028, .0042, .0056, .0070, .0140</span>
 +
<span class="ydata">
 +
  0.31888,
 +
  0.16625, 
 +
  0.10228,
 +
  0.05821, 
 +
  0.03985,
 +
  0.02549, 
 +
  0.01329, 
 +
</span>
 +
<h3>Mutant 5</h3>
 +
<span class="sequence">caatacgcaaaccgcctctccccgcgcgtcggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctccctcatcaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca</span>
 +
<span class="xdata">0, .0014, .0028, .0042, .0056, .0070, .0140</span>
 +
<span class="ydata">
 +
  0.63174,
 +
  0.33524, 
 +
  0.17440,
 +
  0.09337, 
 +
  0.03043,
 +
  0.03337, 
 +
  0.01686, 
 +
</span>
 +
<h3>Mutant 6</h3>
 +
<span class="sequence">caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctctacactttatgcttccggcttgtatgttgtgtggaattgtgagcggataacaatttcacaca</span>
 +
<span class="xdata">0, .0014, .0028, .0042, .0056, .0070, .0140</span>
 +
<span class="ydata">
 +
  0.42290,
 +
  0.29347, 
 +
  0.15733,
 +
  0.09471, 
 +
  0.05778,
 +
  0.03850, 
 +
  0.01475, 
 +
</span>
 +
<h3>Mutant 7</h3>
 +
<span class="sequence">caatacgcaaaccgcctctccccgcgcgttggccgattcattagtgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgtagtgtggaattgtgagcggatatcaatttcacaca</span>
 +
<span class="xdata">0, .0014, .0028, .0042, .0056, .0070, .0140</span>
 +
<span class="ydata">
 +
  0.58989,
 +
  0.32402, 
 +
  0.20047,
 +
  0.11183, 
 +
  0.06365,
 +
  0.03915, 
 +
  0.01712, 
 +
</span>
 +
 +
 +
</div>
 +
</div>
</div>

Revision as of 00:47, 25 September 2011

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Criteria

View our judging criteria for iGEM 2011 here.

Some preliminary data. These are LacI promoter mutants. The green bars represent mutants that are outside of 1.5 standard deviations of wildtype LacI promoter. There's still more to come!

Wildtype

caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca 0, .0014, .0028, .0042, .0056, .0070, .0140 0.99315, 0.78019, 0.58405, 0.45400, 0.35401, 0.29099, 0.13305, 4 0, 1, 2, 5 0.00000, 0.20000, 0.40000, 0.60000, 0.80000, 1.00000, 2.00000 0.99315, 1.02097, 0.99023, 0.99354, 0.78019, 0.97021, 0.96725, 0.96226, 0.58405, 0.87100, 0.89795, 0.94529, 0.45400, 0.76333, 0.85071, 0.93618, 0.35401, 0.65243, 0.75444, 0.88056, 0.29099, 0.55454, 0.68266, 0.84441, 0.13305, 0.26829, 0.37015, 0.55294

Mutant 1

caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca 0, .0014, .0028, .0042, .0056, .0070, .0140 0.68829, 0.48655, 0.27198, 0.15260, 0.08426, 0.05322, 0.01741,

Mutant 2

caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattattgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacgca 0, .0014, .0028, .0042, .0056, .0070, .0140 0.68514, 0.43727, 0.27724, 0.17817, 0.11684, 0.08021, 0.03415,

Mutant 3

caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaaagtgagttagctcactcattaggcaccccaggctttacactttaagcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca 0, .0014, .0028, .0042, .0056, .0070, .0140 0.67026, 0.44322, 0.28504, 0.17543, 0.10938, 0.07325, 0.02937,

Mutant 4

caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgcgcgcaacgcaactaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacgca 0, .0014, .0028, .0042, .0056, .0070, .0140 0.31888, 0.16625, 0.10228, 0.05821, 0.03985, 0.02549, 0.01329,

Mutant 5

caatacgcaaaccgcctctccccgcgcgtcggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctccctcatcaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca 0, .0014, .0028, .0042, .0056, .0070, .0140 0.63174, 0.33524, 0.17440, 0.09337, 0.03043, 0.03337, 0.01686,

Mutant 6

caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctctacactttatgcttccggcttgtatgttgtgtggaattgtgagcggataacaatttcacaca 0, .0014, .0028, .0042, .0056, .0070, .0140 0.42290, 0.29347, 0.15733, 0.09471, 0.05778, 0.03850, 0.01475,

Mutant 7

caatacgcaaaccgcctctccccgcgcgttggccgattcattagtgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgtagtgtggaattgtgagcggatatcaatttcacaca 0, .0014, .0028, .0042, .0056, .0070, .0140 0.58989, 0.32402, 0.20047, 0.11183, 0.06365, 0.03915, 0.01712,

Retrieved from "http://2011.igem.org/Team:UC_Davis/Data_LacI"