Team:Kyoto

From 2011.igem.org

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= '''Carnivorous E.coli''' =
 
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HOMEとPROJECTが重なってると思うので一緒にしませんか
 
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We don't regard E.coli as an animal because they don't hunt or eat.
 
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Then, if it could hunt and eat, will we think it is an animal?
 
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We will create carnivorous E.coli this year.
 
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Carnivorous E.coli can prey on insects when they are hungry.
 
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To explain our project concretely, we made an animation.
 
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<html>
 
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<center>
 
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<iframe width="560" height="350" src="https://static.igem.org/mediawiki/2011/f/f0/CarnivorousEcoli.swf" frameborder="0" allowfullscreen></iframe>
 
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</center>
 
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</html>
 
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In this animation, carnivorous E.coli takes four actions; hunger, luminescence, predation and digestion.
 
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='''iGEM Kyoto'''=
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== Summary ==
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We create new E.coli which hunt and eat insects.
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it is named Carnivorous E.coli.
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Carnivorous E.coli emits light when hungry, and insects come near it attracted by the light.
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Then, it secretes viscous material to catch the insects.
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The insects caught are solved by protease and chitinase that Carnivorous E.coli secretes also.
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So, Carnivorous E.coli can hunt!
== <html><a href="https://2011.igem.org/Team:Kyoto/Hunger">Hunger</a></html> ==
== <html><a href="https://2011.igem.org/Team:Kyoto/Hunger">Hunger</a></html> ==

Revision as of 04:23, 24 September 2011

Contents

iGEM Kyoto

Summary

We create new E.coli which hunt and eat insects. it is named Carnivorous E.coli. Carnivorous E.coli emits light when hungry, and insects come near it attracted by the light. Then, it secretes viscous material to catch the insects. The insects caught are solved by protease and chitinase that Carnivorous E.coli secretes also. So, Carnivorous E.coli can hunt!

Hunger

It is a burden for the E.coli to emit light. This can be reduced by using nitrogen regulatory proteins, NtrB and NtrC, which activate a certain promoter under the condition that supply of nitrogen is not enough.


Luminescence

There are many ways to attract bugs, for instance using pheromone, but it is difficult for E.coli to synthesize complex compounds like pheromone. Carnivorous E.coli emits light and attracts bugs like glowworms by using Bioluciferase from 2010 Cambridge.


Predation

Carnivorous E.coli traps insects by mucilage. Inducing gum gene family, it secretes xanthan gum.

Digestion

Digestion is accomplished by protease and chitinase. We will induce these enzymes and assay them.


Achievement

Human Practice

Reference

Retrieved from "http://2011.igem.org/Team:Kyoto"