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Team:Washington/Protocols/Purified Enzyme Assay
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Revision as of 01:11, 23 September 2011
Purified Enzyme Assay
- Assay
- Add 90uL of 5uM substrate (in NaAc pH 4.0 buffer) to each well of a black fluorescent microtiter assay plate
- Start reaction by adding 0.0125mg/mL enzyme (try to avoid bubbles and pippette quickly, but accurately)
- Use the P20 multichannel with a taped cardboard stopper to make sure you don't hit the pellet!
- Monitor the reaction with the SpectraMax
