Team:Paris Liliane Bettencourt/Notebook/2011/08/20/

From 2011.igem.org

(Difference between revisions)
(Created page with "{{:Team:Paris_Bettencourt/tpl_test}}")
 
Line 1: Line 1:
{{:Team:Paris_Bettencourt/tpl_test}}
{{:Team:Paris_Bettencourt/tpl_test}}
 +
== Babak ==
 +
 +
=== Antibiotics experiment ===
 +
 +
All mixtures were spotted on LB+IPTG plated at 37 degrees and then replicated on plate containing both antibiotics, chloramphenicol and Kanamycin.
 +
 +
*M1 is a mixture of SB463 bacillus strain, harboring chloramphenicol resistance, and SB513 B.subtilis strain, which is resistant to Kanamycin.
 +
 +
*M2 is a mixture of SB463 bacillus strain, which is resistant to chloramphenicol, and K12 E.coli strain (S33), which is resistant to Kanamycin.
 +
 +
*M3 is a mixture of K12 E.coli strain (S34), harboring chloramphenicol resistance, and SB513 B.subtilis strain, which is resistant to Kanamycin.
 +
 +
*M4 is a mixture of K12 E.coli strain(S34), harboring chloramphenicol resistance, and K12 E.coli strain (S33), which is resistant to Kanamycin.
 +
 +
*SB 463, SB 513, S34 and S33 are negative controls
 +
 +
[[File:CP0408_qnt.jpg|thumb|center|Antibiotic Experiments all mixtures ]]
 +
 +
 +
 +
We use 6µg/mL concentration of both Antibiotics and 100µg/mL of IPTG on LB Agar plate.

Latest revision as of 03:52, 22 September 2011

Team IGEM Paris 2011

Babak

Antibiotics experiment

All mixtures were spotted on LB+IPTG plated at 37 degrees and then replicated on plate containing both antibiotics, chloramphenicol and Kanamycin.

  • M1 is a mixture of SB463 bacillus strain, harboring chloramphenicol resistance, and SB513 B.subtilis strain, which is resistant to Kanamycin.
  • M2 is a mixture of SB463 bacillus strain, which is resistant to chloramphenicol, and K12 E.coli strain (S33), which is resistant to Kanamycin.
  • M3 is a mixture of K12 E.coli strain (S34), harboring chloramphenicol resistance, and SB513 B.subtilis strain, which is resistant to Kanamycin.
  • M4 is a mixture of K12 E.coli strain(S34), harboring chloramphenicol resistance, and K12 E.coli strain (S33), which is resistant to Kanamycin.
  • SB 463, SB 513, S34 and S33 are negative controls
Antibiotic Experiments all mixtures


We use 6µg/mL concentration of both Antibiotics and 100µg/mL of IPTG on LB Agar plate.

Retrieved from "http://2011.igem.org/Team:Paris_Liliane_Bettencourt/Notebook/2011/08/20/"