Team:Valencia/Notebook/Week4

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<a class="bannertoplinks" href="https://2011.igem.org/Team:Valencia"><b>Home</b></a>
 
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</li>
 
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        <li>
 
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<a class="bannertoplinks" href="https://2011.igem.org/Team:Valencia/Team"><b>The team</b></a>
 
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                <ul style="z-index:1">
 
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                        <li><a class="bannerlinks" href="https://2011.igem.org/Team:Valencia/Acknowledgements">Acknowledgements & Attributions</a></li>
 
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                        <li><a class="bannerlinks" href="https://2011.igem.org/Team:Valencia/Gallery">Gallery</a></li>
 
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                                    </ul>
 
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</li>
 
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        <li>
 
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<a class="bannertoplinks" href="https://2011.igem.org/Team:Valencia/Project"><b>The project</b></a>
 
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                <ul style="z-index:1">
 
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                        <li><a class="bannerlinks" href="https://2011.igem.org/Team:Valencia/Achievements">Achievements</a></li>
 
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                        <li><a class="bannerlinks" href="https://2011.igem.org/Team:Valencia/Hardware">Hardware</a></li>
 
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                        <li><a class="bannerlinks" href="https://2011.igem.org/Team:Valencia/Project1">Colicin production</a></li>
 
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                        <li><a class="bannerlinks" href="https://2011.igem.org/Team:Valencia/Project2">pH-stat</a></li>
 
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                        <li><a class="bannerlinks" href="https://2011.igem.org/Team:Valencia/Modeling">Modeling</a></li>
 
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<a class="bannertoplinks" href="https://2011.igem.org/Team:Valencia/Parts"><b>BioBricks</b></a>
 
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                <ul style="z-index:1">
 
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                        <li><a class="bannerlinks" href="https://2011.igem.org/Team:Valencia/Notebook/Calender">Notebook</a></li>
 
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<li><a class="bannerlinks" href="https://2011.igem.org/Team:Valencia/Protocols">Protocols</a></li>
 
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        <li>
 
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<a class="bannertoplinks" href="https://2011.igem.org/Team:Valencia/Safety"><b>Human Practices</b></a>
 
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                <ul style="z-index:1">
 
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                        <li><a class="bannerlinks" href="https://2011.igem.org/Team:Valencia/Society">Our project in society</a></li>
 
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                        <li><a class="bannerlinks" href="https://2011.igem.org/Team:Valencia/Safetycode">Deontological code</a></li>
 
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                        <li><a class="bannerlinks" href="https://2011.igem.org/Team:Valencia/Experts">Asking the experts</a></li>
 
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                        <li><a class="bannerlinks" href="https://2011.igem.org/Team:Valencia/Survey">Ethics survey</a></li>
 
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                        <li><a class="bannerlinks" href="https://2011.igem.org/Team:Valencia/Community">Community outreach</a></li>
 
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                        <li><a class="bannerlinks" href="https://2011.igem.org/Team:Valencia/Safetyquestions">Biosafety and Biosecurity</a></li>
 
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                </ul>
 
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<div class=center>
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<div class=col_center_top><b>WEEK 4</b></div><!-- fin clase col_left_top-->
<div class=col_center_top><b>WEEK 4</b></div><!-- fin clase col_left_top-->
<div class=col_center_center>
<div class=col_center_center>
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<b>Hardware</b>: We literally beg in different institutions in order to find a peristaltic pump and a membrane to homogenize the pH.
+
<b>Engineering:</b> We literally beg in different institutions in order to find a peristaltic pump and a membrane to homogenize the pH between different flasks.
-
Finally we find a peristaltic pump that was used in an old hemodialysis machine. Thanks to the hospital!!!!!
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Finally we found a peristaltic pump that was used in an old hemodialysis machine. Thanks to the hospital!!!!!
<br><br>
<br><br>
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<b>pH-stat</b>  
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<b>pH-stat:</b> The containers to be used was prepared and sterilized for the experimental implementation of the first culture, consisting of two 330 ml bottles with a concentration of fertilizer 1 ml / l distilled water, a  12h/12h photoperiod.</p>  
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<br><br>
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<p>Supervision of the evolution of pH and cellular concentration in our cultures, who started working well, increasing the number of cells / ml and the absorbance data was taken at 440 nm.</p>
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<b>Colicin production</b>
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<br>
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<b>Colicin production:</b> We found that antimicrobial peptides such as defensines bring difficulties. We decide it would be better to opt for bacteriocins, easier to manage and control. We begin to document about them. <br>  
  </div><!-- fin clase col_left_top-->
  </div><!-- fin clase col_left_top-->
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Latest revision as of 03:51, 22 September 2011



WEEK 4
Engineering: We literally beg in different institutions in order to find a peristaltic pump and a membrane to homogenize the pH between different flasks. Finally we found a peristaltic pump that was used in an old hemodialysis machine. Thanks to the hospital!!!!!

pH-stat: The containers to be used was prepared and sterilized for the experimental implementation of the first culture, consisting of two 330 ml bottles with a concentration of fertilizer 1 ml / l distilled water, a 12h/12h photoperiod.

Supervision of the evolution of pH and cellular concentration in our cultures, who started working well, increasing the number of cells / ml and the absorbance data was taken at 440 nm.


Colicin production: We found that antimicrobial peptides such as defensines bring difficulties. We decide it would be better to opt for bacteriocins, easier to manage and control. We begin to document about them.
Notebook Week 1 Week 2 Week 3 Week 4 Week 5 Week 6 Week 7 Week 8 Week 9 Week 10 Week 11 Week 12 Week 13 Project

Retrieved from "http://2011.igem.org/Team:Valencia/Notebook/Week4"