Team:EPF-Lausanne/Our Project/T7 promoter variants/selection

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(Results)
(Results)
 
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The colony fluorescence count, which is our other method for quantifying DNA concentration in the supernatant, also confirms  the qPCR data from the previous graph. Indeed, the ratio of RFP colonies to GFP colonies per plate in the case of cells that lyse and release RFP plasmids grows over the course of the experiment. In practical terms, the likelihood of recovering DNA that is not the desired DNA (i.e. natural cell death leading to plasmids in the supernatant) is low.  
The colony fluorescence count, which is our other method for quantifying DNA concentration in the supernatant, also confirms  the qPCR data from the previous graph. Indeed, the ratio of RFP colonies to GFP colonies per plate in the case of cells that lyse and release RFP plasmids grows over the course of the experiment. In practical terms, the likelihood of recovering DNA that is not the desired DNA (i.e. natural cell death leading to plasmids in the supernatant) is low.  
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[[File:flasks_lysis.jpg|600px|center]]
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[[File:flasks_lysis.jpg|thumb|600px|The flasks, after ten hours, show a clear difference in color. The flask on the left contains a lysed culture of RFP-containing co-transformations with a non-lysed culture of GFP-containing co-transformations which accounts for its yellow-green hue. The flask on the right, conversely, contains a lysed culture of the GFP-containing co-transformations with a non-lysed culture of RFP-containing co-transformations, which accounts for its pinkish hue.]]
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The flasks, after ten hours, show a clear difference in color. The flask on the left contains a lysed culture of RFP-containing co-transformations with a non-lysed culture of GFP-containing co-transformations which accounts for its yellow-green hue. The flask on the right, conversely, contains a lysed culture of the GFP-containing co-transformations with a non-lysed culture of RFP-containing co-transformations, which accounts for its pinkish hue. 
 
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Latest revision as of 03:42, 22 September 2011