Team:DTU-Denmark/Data

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[[File:DTU1_Data_page-figure1.png|665px|thumb|center| '''1.''' Tetracycline induces chiX transcription. '''2.''' chiX base-pair and sequester the lacZ mRNA RBS, which inhibits translation of the mRNA. '''3.''' Arabinose induces chiXR transcription. '''4.''' chiXR base-pair to chiX , which promote degradation of the sRNA. '''5.''' LacZ repression is relieved.]]
[[File:DTU1_Data_page-figure1.png|665px|thumb|center| '''1.''' Tetracycline induces chiX transcription. '''2.''' chiX base-pair and sequester the lacZ mRNA RBS, which inhibits translation of the mRNA. '''3.''' Arabinose induces chiXR transcription. '''4.''' chiXR base-pair to chiX , which promote degradation of the sRNA. '''5.''' LacZ repression is relieved.]]
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[[File:DTU1_Data_page-figure2.png|665px|thumb|center|In a strain that constutively expresses chiX, but is lacking chiXR induction with chitobiose only has the effect of inducing transcription, not removing chiX downregulation.]]
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[[File:DTU1_Data_page-figure2.png|665px|thumb|center|In a strain that constutively expresses chiX, but is lacking chiXR, induction with chitobiose only has the effect of inducing transcription, not removing chiX downregulation.]]
[[File:DTU1_Data_page-figure3.png|665px|center|thumb|In a strain deficient in chiX induction with chitobiose has little effect since the chiP RBS is already deregulated.]]
[[File:DTU1_Data_page-figure3.png|665px|center|thumb|In a strain deficient in chiX induction with chitobiose has little effect since the chiP RBS is already deregulated.]]
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Revision as of 02:32, 22 September 2011

Data page

1. Tetracycline induces chiX transcription. 2. chiX base-pair and sequester the lacZ mRNA RBS, which inhibits translation of the mRNA. 3. Arabinose induces chiXR transcription. 4. chiXR base-pair to chiX , which promote degradation of the sRNA. 5. LacZ repression is relieved.
In a strain that constutively expresses chiX, but is lacking chiXR, induction with chitobiose only has the effect of inducing transcription, not removing chiX downregulation.
In a strain deficient in chiX induction with chitobiose has little effect since the chiP RBS is already deregulated.

Data For Our Favorite New Parts:

  1. [http://partsregistry.org/Part:BBa_K564012 Main Page] PchiP(original):lacZ, BBa_K564012 : Promoter of the chitoporin chiP gene fused with lacZ (from [http://partsregistry.org/Part:BBa_I732005 BBa_I732005]) encoding β-galactosidase. Is induced by chitobiose and is regulated by chiX
  2. [http://partsregistry.org/Part:BBa_K564013 Main Page] PchiP(mutated):lacZ, BBa_K564013: Mutated promoter of the chitoporin chiP gene fused with lacZ (from [http://partsregistry.org/Part:BBa_I732005 BBa_I732005]) encoding β-galactosidase. Is induced by chitobiose, but not regulated by chiX

Data For Pre-existing Parts:

  1. [http://partsregistry.org/Part:BBa_I732005:Experience Experience] lacZ (encoding beta-galactosidase, full-length), BBa_I732005: This part controlled by the chiP promoter gave blue colonies when grown on chitobiose.
  2. [http://partsregistry.org/Part:BBa_I746908:Experience Experience] Inducible pBad/araC promoter, BBa_I746908: We generated an synthetic promoter library (SPL) based on this part. This allows for the generation of a vast number of arabinose inducible promoters of various strengths.
The arabinose promoter was improved both by rationally changing the -10 and the -35 part of the promoter, and randomly by generating an SPL from the wild-type promoter.