Team:EPF-Lausanne/Our Project/T7 promoter variants/t7prom

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We made two families of T7 promoter variants. Each family has six designed mutants and three randomer mutants. The only difference between the two families is the addition of a lac operator sequence downstream of the T7 promoter.  
We made two families of T7 promoter variants. Each family has six designed mutants and three randomer mutants. The only difference between the two families is the addition of a lac operator sequence downstream of the T7 promoter.  
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[[File:t7_lac_rbs_lysis_term.png|400px|center]]
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[[File:t7_lac_rbs_lysis_term.png|470px|center]]
[[File:t7_rbs_lysis_term.png|400px|center]]
[[File:t7_rbs_lysis_term.png|400px|center]]
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These promoter variants were characterized using fluorescence, revealing a wide range of promoter efficiencies.  
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These promoter variants were characterized using fluorescence, revealing a wide range of promoter efficiencies. To find out more about how these T7 promoter variants were made, please click [[Team:EPF-Lausanne/Our Project/T7 promoter variants/t7prom/making|here]].
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Here is a version of the T7 promoter consensus sequence:
Here is a version of the T7 promoter consensus sequence:
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===Non-random T7 promoter variants===
===Non-random T7 promoter variants===
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The T7 promoter variants in this section were designed based on considerations from literature. These sequences, and their corresponding predicted strengths, also come from literature. The exact source is Imburgio, D., Rong, M., Ma, K. & McAllister, W.T. Studies of promoter recognition and start site selection by T7 RNA polymerase using a comprehensive collection of promoter variants. Biochemistry 39, 10419–10430 (2000).  
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The T7 promoter variants in this section were designed based on considerations from the relevant literature. These sequences, and their corresponding predicted strengths, also come from literature. The exact source is Imburgio, D., Rong, M., Ma, K. & McAllister, W.T. Studies of promoter recognition and start site selection by T7 RNA polymerase using a comprehensive collection of promoter variants. Biochemistry 39, 10419–10430 (2000).  
Variants without additional lac operator (constitutive):
Variants without additional lac operator (constitutive):

Latest revision as of 02:11, 22 September 2011