Team:Paris Bettencourt/Preliminaries

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<h1>Demonstrative experiments</h1>
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<p>The Dubey and Ben-Yehuda work <a href="http://bms.ucsf.edu/sites/ucsf-bms.ixm.ca/files/marjordan_06022011.pdf">[1]</a> present a set of  experiments to demonstrate the existence of the nanotubes. We reproduced some of them in order to demonstrate again the existence of these entities as a medium of communication between bacteria, and to be sure we are in the good experimental conditions to reproduce them as basis for testing our designs.</p>
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<p>We reproduced the two keystone experiments of the paper: the GFP diffusion, and the antibiotic resistance exchange  (with less suscess).</p>
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  <td style="width:200px; text-align:center"><a href="https://2011.igem.org/Team:Paris_Bettencourt/GFP_diff"><img style="width:150px" src="https://static.igem.org/mediawiki/2011/8/80/GFP-diff-button.png"></a>
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  <td><b>The <a href="https://2011.igem.org/Team:Paris_Bettencourt/GFP_diff">GFP diffusion experiment</a></b> is a simple experiment. One Bacillus Subtlis strain that produces GFP is mixed with a wild type strain on solid support under the microscope. If some nanotubes are formed, the GFP will diffuse through the tubes and color the non fluorescent strain. We invite you to <a href="https://2011.igem.org/Team:Paris_Bettencourt/GFP_diff">visit the corresponding page</a> to learn more about what we did and see our successful reproduction of this set of results.
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  <td style="width:200px; text-align:center"><a href="https://2011.igem.org/Team:Paris_Bettencourt/Atb_exp"><img style="width:150px" src="https://static.igem.org/mediawiki/2011/2/2b/Question_mark_button.png"></a>
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  <td><p><b>The <a href="https://2011.igem.org/Team:Paris_Bettencourt/Atb_exp">antibiotic resistance exchange</a></b> is a more tricky experiment in which bacteria are sought to exchange resistance enzyme(s) (or their coding mRNA) through nanotubes, thus allowing a mixed population of two strains, each carrying a different resistance gene to survive on agar plate with both antibiotics incorporated.We invite you to <a href="https://2011.igem.org/Team:Paris_Bettencourt/Atb_exp">visit corresponding the page</a> to learn more about what we did and the mixedresults we had, suggesting alternative explanation to the survival of the two populations.</p>
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<p>In the Ben-Yehuda paper <a href="http://bms.ucsf.edu/sites/ucsf-bms.ixm.ca/files/marjordan_06022011.pdf">[1]</a> a set of simple experiments are made to prove the existence of the nanotubes. We tryied to reproduce some of them in order to demonstrate again the existence of these entities as a medium of communication between bacteria, and to be sure we are in the good experimental conditions to produce them and that we can go on with our designs.</p>
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<p>We tryied to reproduce the two keystone experiments of the paper: the GFP diffusion, and the antibiotic resistance exchange, with less sucess for the latter.</p>
 
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<p><b>The <a href="https://2011.igem.org/Team:Paris_Bettencourt/GFP_diff">GFP diffusion experiment</a></b> is the simplest experiment possible. One Bacillus Subtlis strain that produce GFP is mixed with a wild type strain. If some nanotubes are formed, the GFP will diffuse through the tubes and color the non fluorescent strain. We invite you to <a href="https://2011.igem.org/Team:Paris_Bettencourt/GFP_diff">visit corresponding the page</a> to learn more about what we did and the results we had.</p>
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<p><b>The <a href="https://2011.igem.org/Team:Paris_Bettencourt/Atb_exp">antibiotic resistance exchange</a></b> is a more tricky experiment in which bacteria are shown to exchange resistance enzyme through nanotube and allow the population to survive even though all the cells does not carry the resistence.</p>
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Latest revision as of 01:38, 22 September 2011

Team IGEM Paris 2011

Demonstrative experiments

The Dubey and Ben-Yehuda work [1] present a set of experiments to demonstrate the existence of the nanotubes. We reproduced some of them in order to demonstrate again the existence of these entities as a medium of communication between bacteria, and to be sure we are in the good experimental conditions to reproduce them as basis for testing our designs.

We reproduced the two keystone experiments of the paper: the GFP diffusion, and the antibiotic resistance exchange (with less suscess).

The GFP diffusion experiment is a simple experiment. One Bacillus Subtlis strain that produces GFP is mixed with a wild type strain on solid support under the microscope. If some nanotubes are formed, the GFP will diffuse through the tubes and color the non fluorescent strain. We invite you to visit the corresponding page to learn more about what we did and see our successful reproduction of this set of results.

The antibiotic resistance exchange is a more tricky experiment in which bacteria are sought to exchange resistance enzyme(s) (or their coding mRNA) through nanotubes, thus allowing a mixed population of two strains, each carrying a different resistance gene to survive on agar plate with both antibiotics incorporated.We invite you to visit corresponding the page to learn more about what we did and the mixedresults we had, suggesting alternative explanation to the survival of the two populations.

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