Team:Paris Bettencourt/Experiments/Methodologies/K090403J

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After having used a replicative plasmid (pHM3), we tried to use a Bacillus subtilis integration plasmid. For that, we used and plan to continue the construction of the B.subtilis integration vector pSBINT1 built by the 2008 Cambridge iGEM team.
 
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== Cyrille ==
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Control digestion of pHM3 in NcoI
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[[File:CP2009.jpg|thumb|center]]
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Control of the size of the insert
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[[File:CP2009_XP.jpg|thumb|center|S75 *5; S82 *5; 104 *5]]
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[[File:CP2009_XP_ComS.jpg|thumb|center|ComS *3]]
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== Baptiste, Hovannes & Ouriel ==
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===Characterisation of T7 autoloop, RFP - GFP ===
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We characterized T7 autoloop by microscopy.
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Leak,
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TEXT
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{| border="1" class="wikitable" style="text-align: center;"
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|+T7 autoloop full no terminator / E-coli at 37°C
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|-
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|[[File:T7 autoloop ctrl-25.jpg|290px|thumb|center|E-Coli  at 37°C  (trans image)]]
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|[[File:T7 autoloop ctrl-26.jpg|290px|thumb|center|E-Coli  at 37°C  (gfp image)]]
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|[[File:T7 autoloop ctrl-27.jpg|290px|thumb|center|E-Coli  at 37°C  (rfp image)]]
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|-
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|[[File:T7 autoloop ctrl-31.jpg|290px|thumb|center|E-Coli  at 37°C  (trans image)]]
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|[[File:T7 autoloop ctrl-32.jpg|290px|thumb|center|E-Coli  at 37°C  (gfp image)]]
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|[[File:T7 autoloop ctrl-33.jpg|290px|thumb|center|-Coli  at 37°C  (rfp image)]]
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|}
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{| border="1" class="wikitable" style="text-align: center;"
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|+T7 autoloop full with  terminator / E-coli at 37°C
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|-
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|[[File:T7_loop_1.jpg|290px|thumb|center|E-Coli  at 37°C  (trans image)]]
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|[[File:T7 loop 1 gfp.jpg|290px|thumb|center|E-Coli  at 37°C  (gfp image)]]
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|[[File:T7 loop 1 rfp.jpg|290px|thumb|center|E-Coli  at 37°C  (rfp image)]]
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|-
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|[[File:T7 loop 2.jpg|290px|thumb|center|E-Coli  at 37°C  (trans image)]]
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|[[File:T7 loop 1 gfp.jpg|290px|thumb|center|E-Coli  at 37°C  (gfp image)]]
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|[[File:T7 loop 2 rfp.jpg|290px|thumb|center|-Coli  at 37°C  (rfp image)]]
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|}
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===YFP-tetR===
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We
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TEXT
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Revision as of 01:38, 22 September 2011

Team IGEM Paris 2011


Contents

Cyrille

Control digestion of pHM3 in NcoI

CP2009.jpg

Control of the size of the insert

S75 *5; S82 *5; 104 *5
ComS *3

Baptiste, Hovannes & Ouriel

Characterisation of T7 autoloop, RFP - GFP

We characterized T7 autoloop by microscopy. Leak,


T7 autoloop RFP+ Control / E-coli at 37°C
E-Coli at 37°C (trans image)
E-Coli at 37°C (gfp image)
E-Coli at 37°C (rfp image)
E-Coli at 37°C (trans image)
E-Coli at 37°C (gfp image)
-Coli at 37°C (rfp image)


TEXT


T7 autoloop full no terminator / E-coli at 37°C
E-Coli at 37°C (trans image)
E-Coli at 37°C (gfp image)
E-Coli at 37°C (rfp image)
E-Coli at 37°C (trans image)
E-Coli at 37°C (gfp image)
-Coli at 37°C (rfp image)


T7 autoloop full with terminator / E-coli at 37°C
E-Coli at 37°C (trans image)
E-Coli at 37°C (gfp image)
E-Coli at 37°C (rfp image)
E-Coli at 37°C (trans image)
E-Coli at 37°C (gfp image)
-Coli at 37°C (rfp image)

YFP-tetR

We


TEXT

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